Name: Flow Cytometry Antibody CD34-PerCP-Cyanine5.5, Clone 581
- Antibody CD34-PerCP-Cyanine5.5 is a monoclonal antibody (mAb) labelled with Peridin chlorophyll protein-Cyanine 5.5 (PerCPCyanine5.5), designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD34 antigen by Flow Cytometry (FC). This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
CD34 is a type I monomeric sialomucin-like glycophophoprotein with an approximate molecular weight of 105-120 kD. It is expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. CD34 antigen is not expressed in normal peripheral blood lymphocytes, monocytes, granulocytes, and platelets. Its density is highest on early hematopoietic progenitor cells and decreases as mature cells. The antigen is absent on fully differentiated hematopoietic cells. Uncommitted CD34+ progenitor cells are CD38- and lack lineage-specific antigens such as CD71, CD33, CD10, and CD5, while CD34+ cells that are lineage-committed express the CD38 antigen in high density. Most CD34+ cells express either the CD45RO or CD45RA antigens, with the CD45RO+ population being the more primitive. CD34 antigen is expressed in 60% of acute B-lymphoid leukemias and acute myeloid leukemias and in 1% to 5% of acute T-lymphoid leukemias but is not expressed on chronic lymphoid leukemias or lymphomas.
The purified monoclonal CD34 antibody conjugated with the tandem PerCP-Cyanine5.5 is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.
- Clone: 581.
- Isotype: Mouse / IgG1.
- Amount per vial: 50 tests (3 µL mAb per determination).
- Reagent is considered non-sterile.