Name: Flow Cytometry Antibody CD56-PE, Clone C5.9
- Antibody CD56 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD56 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
The anti-CD56 mAb recognizes the CD56 antigen present on NK cells and subsets of CD3+CD4+ and CD3+CD8+ T-cells, and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc .
The NK cells (CD3-CD56+) count is expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by FC based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.
REAGENT COMPOSITION
Purified monoclonal antibody anti-CD56 conjugated with different fluorochromes (see table above), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.
- Clone: C5.9
- Isotype: Mouse / IgG2b
- Purification: Affinity chromatography
- Size: 200 tests (1 ml per vial) Usage: 5 µl mAb per determination.
- Reagents are not considered sterile.
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