Name: Flow Cytometry Antibody CD59-FITC, Clone MEM-43
- Antibody CD59-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD59 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Membrane-associated C-regulatory proteins are the major protection against Complement attack. CD59 (Protectin), a 19 kDa GPI-linked membrane glycoprotein is one of these regulatory proteins binding to the complement components C8 and C9 preventing the destruction of autologous cells. CD59 has a broadly distributed expression on all lymphocytes, monocytes, granulocytes, erythrocytes and in a variety of non-haematological cells. CD59 modulates T cell adhesion and plays a role in T cell activation. CD59 deficiency was already detected in clinical inflammatory disorders. Cells from patients with the haematological disorder paroxysmal nocturnal haemoglobinuria (PNH) are defective for GPI anchor synthesis and hence lack surface expression of CD59 and are highly susceptible to the lytic effects of Complement attack. For this reason CD59 together with CD55, another GPI-anchored protein, have been systematically used in diagnosis of PNH.
The purified monoclonal CD59 Antibody conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: MEM-43
- Isotype: IgG2a.
- Amount per 1 mL vial: 200 tests (5 µL mAb per determination).
- Reagent is considered non-sterile.