Name: Flow Cytometry Antibody CD62L-FITC, Clone SK11
- Antibody CD62L-FITC is a monoclonal antibody (mAb) labelled with the tandem fluorescein isothiocyanate (FITC), designed for flow cytometry (FC) use as direct immunofluorescence reagent for the identification and enumeration of CD62L antigenexpressing cells. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
CD62L is a single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer’s patches. It also plays a role in leukocyte rolling on activated endothelial cells.
Purified monoclonal CD62L Antibody conjugated with the tandem fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide and 0,2% (w/v) bovine serum albumin (BSA).
- Clone: SK-11
- Isotype: Mouse / IgG2a
- Purification: Affinity chromatography
- Amount per vial: 100 tests (5 µL mAb)
- Reagent is not considered sterile.