Name: Flow Cytometry Antibody CD8-APC, Clone 17D8
Ø Antibody CD8-APC is a monoclonal antibody (mAb) labelled with allophycocyanin (APC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD8 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. CYT-8AP mAb recognizes CD8 antigen present on suppressor/cytotoxic T cells (CD3+CD8+) and can therefore be used in lymphocytes immunophenotyping. These studies are widely applied when monitoring post-transplant patient’s immunologic status and when characterizing and following-up immunodeficiencies, autoimmune diseases, leukaemia, etc.
The purified monoclonal CD8 antibody conjugated with allophycocyanin (APC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
Amount per vial: 200 tests (5 µl mAb per determination).
Reagent is considered non-sterile.