Name: Flow Cytometry Antibody HLA-B7-PE, Clone BB7.1
- HLA-B7-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of HLA-B7 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. CYT-HLAIB7PE binds to the HLA-B7 antigen and does not cross react with HLAB27 or other related HLA antigens. HLAB27 and HLA-B7 antigens are expressed in 7% and 22% of individuals of Caucasian origin. This mixture of antibodies permits the characterization of HLAB27 specificity in the HLA class I allotype in patients suffering from inflammatory disorders affecting the sacroiliac and intervertebral joints. HLAB27-PE antibody can be used to distinguish true HLA-B27 positives (HLA-B27+ /HLA-B7- ) from false HLA-B27 positives (HLAB7dim+/HLA-B7+).
The purified monoclonal HLA-B7 Antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: BB7.1
- Isotype: IgG1.
- Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.