Name: Flow Cytometry Antibody HLA-DR-Pacific BlueTM, Clone L243
- HLA-DR is a monoclonal antibody (mAb) labelled with Pacific BlueTM designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of HLA-DR antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. The main function of human leucocyte antigen (HLA) molecules is to present antigenic peptides to the T-cell receptor, thereby regulating the induction of the immune response. The HLA molecules are encoded by a cluster of tightly linked genes located on the short arm of chromosome 6. Three classes of HLA molecules (I, II and III) have been denoted. Human class II genes are located in the HLA-D region, consisting of three families called DQ, DP and DR. The products of class II genes form a heterodimeric transmembrane protein, consisting of a heavy α-chain and a light βchain. The DR α-chain is expressed from one non-polymorphic gene, whereas the DR β- chain originates from nine highly polymorphic genes. HLA-DR antigen is constitutively expressed on antigen-presenting cells, such as B lymphocytes, monocytes and dendritic cells but can also be detected on activated T lymphocytes and activated granulocytes. The antigen has been found expressed in cases of different types of acute lymphoblastic leukaemias, acute myeloid leukaemias, chronic lymphoblastic leukaemias, chronic myeloid leukaemias and B- and T-cell non-Hodgkin’s leukaemias. However, the antigen is normally not present on non-haematopoietic tumours and multiple myelomas.
The purified monoclonal HLA-DR Antibody conjugated with Pacific BlueTM is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.
- Clone: L243
- Isotype: IgG2a.
- Amount per vial: 100 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.