Name: Flow Cytometry Antibody TdT-FITC, Clone Polyclonal
- Antibody TdT-FITC is a polyclonal antibody (pAb) labelled with fluorescein isothiocyanate (FITC) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the Terminal deoxynucleotidyl Transferase (TdT) by flow cytometry (FC). TdT is an intracellular marker for immature lymphocytes in the hemapoietic system and is considered essential for the initial evaluation of acute leukaemia’s together with a panel of other antibodies.
SUMMARY AND EXPLANATION
TdT is a 58 kDa protein that catalyses the polymerization of deoxynucleotides on the 3’ hydroxyl end of a polydeoxynucleotide without template instructions. TdT is present in the nuclei of normal T and B lymphocyte precursors and the neoplastic equivalent. Positive cells are present in normal thymus, and in few normal bone marrow cells, corresponding to hematopoietic precursors. Most cases of ALL are TdT positive, 37 % of AML cases are TdT positive.
REAGENT COMPOSITION
Purified polyclonal TdT Antibody conjugated with fluorescein isothiocyanate (FITC), is supplied in phosphate-buffer saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.
- Isotype: IgG1 + IgG2a.
- Amount per 0,5 mL vial: 50 tests (10 µL/ test).
- Reagents are not considered sterile.
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