Name: Flow Cytometry Antibody CD3-Pacific Blue, Clone UCHT-1
- Antibody CD3-Pacific BlueTM is a monoclonal antibody (mAb) labelled with Pacific BlueTM designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. CYT-3PBZ mAb recognizes the CD3 antigen present in T cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. Pacific BlueTM, is excited with the violet laser (405nm) and emits at 550nm. This fluorochrome provides maximum resolution and narrow emission peaks, which results in little spectral overlap and minimal compensation requirements. In multicolor panel it is recommended the use of this fluorochrome combined with Orange Cytognos 515, which is also excited with the violet laser and emits at 515nm.
Purified monoclonal CD3 antibody antibody conjugated with Pacific BlueTM, supplied in phosphate buffered saline with 0, 09% sodium azide.
- Clone: UCHT1.
- Isotype: Mouse / IgG1.
- Amount per 0,5 mL vial: 100 tests (5 µL mAb per determination).
- Reagents are not considered sterile.