آنتی بادی مونوکلونال فلوسایتومتری CD117-PE ، کلون 104D2

Name: Flow Cytometry Antibody CD117-APC, Clone 104D2

Antibody CD117 is a mAb designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of CD117 antigen-expressing cells. CD117 is a specific marker to detect leukemic cells committed to the myeloid lineage and therefore it represents a useful reagent in the characterization of acute leukaemias. C-kit expression would be particularly relevant for the diagnosis of biphenotypic acute leukaemias as well since in these cases its association with myeloid lineage is greater than that of the CD13 and CD33 antigens.

CD117 reacts specifically with human c-kit gene product (SCF receptor). The c-kit proto-oncogen (CD117) has been shown to be present in several cell types including normal and neoplastic haemopoietic cells. The majority of CD117+ bone marrow (BM) cells (50-70%), which mainly correspond to myeloid precursors, coexpress the progenitor associated CD34 antigen. The c-kit+/CD34- cells mainly consist of immature cells, mast cells and CD34- erythroid precursors.  In acute leukaemias CD117 expression was initially associated with AML. Nevertheless, at present it is well established that CD117 expression may also be found in a relatively important proportion of T-ALL while it is usually absent in B-lineage ALL. The analysis of CD117 could be relevant for the investigation of minimal residual disease (MRD) since in combination with other antigens may be useful for the identification of leukaemia associated phenotypes in patients who achieved morphological complete remission. The combination of CD117 and other myeloid-associated antigens such as CD11b and CD15 may be of great help to monitoring MRD in AML patients although it defines a subpopulation of myeloid cells which are either absent or present at very low frequencies in normal human BM . Additionally, some acute leukaemias express CD117 over-expression which is not detected in normal cells. Moreover, different studies have shown that in around one-third of both multiple myeloma cases and patients with monoclonal gammopathy of undetermined significance myelomatous plasma cells display reactivity for CD117 .BM mast cells are clearly identifiable on the basis of their light scatter properties and strong CD117 expression. BM mast cells studies result interesting for the diagnosis of adult indolent systemic mast cell disease. and mast cell leukaemia.

REAGENT COMPOSITION

The purified monoclonal CD117 antibody (mAb) conjugated with one fluorochrome (see table above) and supplied in phosphate buffered saline with <0,1% (m/v) sodium azide.

• Clone: 104D2
• Isotype: IgG1
• Amount per 1 ml vial: 100 tests (10 µl mAb per determination)
• Reagent is considered non-sterile.