آنتی­ بادی CD21 (2G9)

Name: CD21 Antibody Clone 2G9

Description and applications: The CD21 or CR2 antigen is a 145 kD glycoprotein of molecular weight, which constitutes one of the
membrane molecules involved in the transduction of growth signals into the B cells. Also acts as a receptor for the Epstein Barr virus. This antibody also reacts with a leukocyte membrane epitope (CR2), with molecular weights of 95, 72, 50, 32 and 28 kD. The 28 and 72 kD molecular weight fragments of CR2 constitute the point of attachment of C3d. CD21 antigen is an antigen that is expressed in mature B cells. The antigen is present with a high density in follicular dendritic cells (CFD) of B zones of lymphoid tissue. CD21 moderately stains B cells and strongly follicular dendritic cells in frozen tissue sections, while staining of B cells is reduced or non existent in the paraffin embedded sections. Immunostaining of CFDsin paraffin-embedded sections is as strong as that of frozen tissue sections. CD21 immunohistological analysis of CFD of non- Hodgkin’s lymphomas in paraffin embedded sections reveals the generally dense and highly defined nodular network of CFD in follicular lymphomas and a diffuse, disorganised network in some types of diffuse lymphomas. B-cell precursor lymphomas (lymphoblastic lymphomas), Burkitt’s lymphomas, plasmacytomas, and hairy cell leukemias are
constantly lacking a CFD. In non-Hodgkin’s lymphomas of T cell origin, CFDs are mainly restricted to peripheral T-cell lymphomas of the
angioinmunoblastic type and some cases of pleomorphic lymphomas. T-cell lymphoblastic lymphomas, T-CLL and mycosis fungoides do not have a CFD network. The only category of Hodgkin’s disease that has a CFD-like network is the nodular lymphocyte-predominant
form.

Composition: Anti-human CD21 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant human CD21.