دسته بندی: کیت های تشخیص
-
Dual StainingMASTER DUAL STAINING KIT
Available Size : 100 test
Intended Use : Diagnostic in vitro in humans
Description: Master Dual Staining Kit contains reagents to perform manual or automated immunohistoquemical dual
staining on human tissue sections, fixed in buffered formalin and embedded in paraffin.
This kit contains a visualization system based on micropolymers and is sufficient to perform 100 determinations
following the recommended protocol.Limitations Of The Reactives
The use on frozen tissue has not been evaluated.
In order to use the Master Dual Staining kit it should be noted that the two antibodies must come from different
animals (mouse / rabbit).
Due to the presence of a soluble chromogen in alcoholic solutions, the slides must be air-dried and for final mounting
an aqueous mounting media should be used. -
APMASTER POLYMER PLUS ALKALINE PHOSPHATASE DETECTION SYSTEM
Available Size : 100 test / 500 test
Kit components:
DAB Substrate Buffer (READY-TO-USE)
DAB Chromogen Concentrate (READY-TO-USE)
DAB enhancer (READY-TO-USE)Master Polymer Plus AP is the result of the experience of a decade in systems of polymers for
immunohistochemistry. The detection system has proven greater sensitivity with an increase in the antigenantibody
binding signal. The new technology of micropolymers provides greater advantages as compared to
the conventional immunohistochemistry techniques and facilitates the detection of antigens in the different
cellular compartments (nucleus, cytoplasm, cytoplasmic membrane) due to the smaller size of the molecule.
The technical procedure used eliminates the background staining originated by the non-specific binding to
endogenous biotin molecules, as Master Polymer Plus AP is a procedure not based in the reaction
avidin/biotin.
The detection system Master Polymer Plus AP is highly sensitive, provides a low background staining and
the results obtained are higher than the ones obtained with the conventional procedures of
Streptavidin/Biotin or long polymers.
The system Master Polymer Plus AP is elaborated with the technology of micropolymers.
This system can be used with:
– Monoclonal primary antibodies obtained in mouse.
– Monoclonal and polyclonal primary antibodies obtained in rabbit. -
DABImmunoperoxidase DAB Kit (Dark Brown)
Available Size : 100 test / 500 test
Kit components:
DAB Substrate Buffer (READY-TO-USE)
DAB Chromogen Concentrate (READY-TO-USE)
DAB enhancer (READY-TO-USE)Spcificity, Interferences and Limitations:
The Immunoperoxidase DAB Kit (Dark Brown) is preferred for immunohistochemical staining methods, based
on peroxidase marked antibodies and in situ hybridization.
Once the DAB oxidation has occurred, a brown insoluble precipitate is obtained. An increase or decrease in the
ratio between the chromogen and its substrate volumes, as well as exposure to bright light, can affect the
degree of specific staining and background of the final reaction. For this reason it is recommended that the
staining should be made in low light conditions. -
DABMaster Polymer Plus Detection System (Peroxidase)
Available Size : 100 test / 500 test / 1250 test
Intended use: Product for in vitro diagnostic use.
Master Polymer Plus Detection System is developed based on the micro-polymer technology and is the result of a decade of experience in polymer systems for immunohistochemistry. This detection system has demonstrated higher sensitivity with an increase in the antigen-antibody binding signal. The novel technology of micro-polymers provides greater advantages than conventional immunohistochemical techniques and facilitates the detection of antigens in different cellular compartments (nucleus, cytoplasm and cell membrane) due to the smaller size of the molecule. It also eliminates background staining caused by nonspecific binding of endogenous biotin molecules as the Master Polymer Plus Detection System staining procedure is not based on the avidin / biotin reaction.
– Primary monoclonal and polyclonal antibodies obtained in mouse
– Primary monoclonal and polyclonal antibodies obtained in rabbit. -
Dual StainingP16INK4/Ki67 Dual Staining Kit
Available Size : 100 test
Intended Use : Diagnostic in vitro in humans
Description: p16INK4/Ki67 Dual Staining Kit is a immunohistochemical staining kit based on the dual staining technology for the simultaneous detection of the p16 protein and the Ki67 proliferative activity marker. The kit has been validated both in cytological samples and in formalin-fixed paraffin embedded-tissue sections using manual or semiautomatic protocol in
LabVision Autostainer type platforms. The co-expression of the two markers p16INK4 and Ki67 is a marker for active/in progress HPV infections. The kit has diagnostic value in lesions caused by the HPV in the female genital tract, especially in differentiating high-grade squamous dysplastic lesions from those with a similar morphology; it can be used in other related lesions. This kit contains reagents for the detection of the antigens p16INK4/Ki67 based on a system of micro-polymers conjugated with peroxidase and alkaline phosphatase, and it is sufficient to perform 100 determinations following the recommended protocol.