دسته: ایمونو هیستوشیمی

Specification:

Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and squamous epithelium. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms, e.g. Schwannomas. The absence of EMA can also be of value since negative EMA staining is characteristic of some tumors including adrenal carcinoma, seminomas, paraganglioma and hepatoma.

Immunogen:

Delipidated extract of human milk fat globule membranes

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

CD21 antigen is an integral membrane glycoprotein of molecular weight 140 kD. Anti-CD21 is useful in the identification of follicular dendritic cell matrix found in normal lymph nodes and tonsillar tissue. This antibody also labels follicular dendritic cell tumor/sarcomas. The antigen is absent on T-lymphocytes, monocytes, and granulocytes. Associated products: CD35, Follicular Dendritic Cell

Presentation:

Anti-CD21 is a rabbit monoclonal from tissue culture supernatant diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide

Specification:

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120–150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70–80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Immunogen:

Crude human uterus extract

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. It is superb for staining formalin-fixed, paraffin-embedded B-cell lymphomas.

Immunogen:

A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein

Presentation:

Bioreactor Concentrate with 0.05% Azide.

Specification:

Napsin A is an aspartic protease with a molecular weight of approximately 38 kDa, expressed in type-II pneumocytes and is involved in the N- and C-terminal processing of proSP-B in type-II pneumocytes. TAO2 has been shown to be identical with Napsin A. There is also a Napsin B gene which is transcribed exclusively in cells related to the immune system but lacks a stop codon and may represent a transcribed pseudogene. Napsin A is predominantly expressed in the lung and kidney. In the lung, Napsin A is expressed in alveolar type II pneumocytes, regulated by TTF-1, and is involved in the generation of the surfactant protein B. Intra-alveolar macrophages contain Napsin A as a result of phagocytosis. In the kidney, Napsin A is expressed in the proximal tubules, where it is involved in lysosomal protein catabolism.

Presentation:

Liquid tissue culture supernatant containing 15mM sodium azide

Specification:

ECAD (syn, CD325), 120 kDA, chromosome 16q22.1 (CDH1 gene), is a critical regulator of epithelial junction formation. It interacts with the cytoskeleton through several associated proteins. The ECAD internal domain binds with alpha, beta, gamma and p120 catenin’s to anchor the ECAD complex to the actin cytoskeleton of the cell. ECAD is expressed in virtually all epithelial cells except for adrenocortical cells. The expression in liver cells is weaker than in most other epithelia. ECAD is also expressed in melanocytes (adhering to squamous epithelial cells).

Presentation:

Tissue culture supernatant containing 15mM sodium azide

Specification:

Cytokeratin’s (epithelial keratins) are an important component of the intermediate filament system. They are mainly insoluble molecules playing an important role in cellular mechanics. There are two types of cytokeratin’s: a) type I: (9-20) keratins that are relatively acidic and bearing a small molecular weight (40-56.5 kDa) and b) type II: (1-8) that are relatively basic-neutral of larger molecular weight (53-67 kDa). Proliferating cells have a substantial pool of two soluble cytokeratin’s, namely CK8 and CK18 and their concentration is high during the G2-M phase of the cell cycle. During apoptosis, CK18 are cleaved by caspases at position Asp396 producing relatively stable fragments.

Presentation:

Tissue culture supernatant containing 15mM sodium azide

Specification:

NK-1 antibody marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. NK-1 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor, and medulloblastoma. NK-1 reportedly also reacts with a variety of cell types in non-lymphoid tissues, including neurofibroma, ganglioneuroma, and prostate carcinoma.

Immunogen:

Human peripheral blood mononuclear cells

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Immunogen:

Human breast carcinoma

Presentation:

Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide