دسته: ایمونو هیستوشیمی

Specification:

This antibody recognizes cytokeratin polypeptides ranging from 40 through 58kD, corresponding to cytokeratin’s 5, 6, 8,17, and 19. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts, and tracheal epithelium.

Immunogen:

Crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells

Presentation:

This antibody is supplied as tissue culture supernatant containing sodium azide as a preservative

Specification:

Ep-CAM/Epithelial Specific Antigen consists of two glycoproteins, 34 and 39 kDa, sometimes designated Epithelial antigen, epithelial specific antigen, and epithelial glycoprotein. In paraffin sections, the protein is detected with mAbs like Ber-EP4 and MOC-31. The glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells except for most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. However, focal positivity may be seen in the basal layer of squamous cell epithelium of endoderm (e.g., palatine tonsils) and mesoderm (e.g., uterine cervix). In liver lesions like hepatitis and cirrhosis, the hepatocytes frequently become Ep-CAM positive. Normal mesothelial cells are Ep-CAM negative but may express focal reaction when undergoing reactive changes. Mesenchymal cells and cells from the neural crest are negative, except for olfactory neurons. Ep-CAM is found in most adenocarcinomas of most sites (50-100% in various studies; as well as neuroendocrine tumours, including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain in about 30% of the cases. Squamous cell carcinoma of endoderm and mesoderm is usually Ber-EP4 positive, while that of ectoderm is negative. Basal cell and basosquamous carcinoma are Ber-EP4 positive in almost all cases. Choroid plexus papilloma and carcinoma are usually negative. Malignant mesothelioma (epithelioid and biphasic) is Ep-CAM positive in 4-26% of the cases. The staining is usually focal but may occasionally be widespread. Synovial sarcoma (epithelioid and biphasic) and desmoplastic small round cell tumour stain for Ep-CAM in most cases. Seminoma, embryonal carcinoma, yolk sac tumour and choriocarcinoma reveal Ber-EP4 positivity in a minor proportion of cases. Among neural tumours, Ep-CAM positivity is seen only in olfactory neuroblastoma. Ep-CAM can be of great help in the differential diagnosis of malignant involvement in the peritoneal and pleural cavities. The lack of reactivity in the majority of malignant mesothelioma can in an appropriate panel be utilized in the discrimination between this tumour and adenocarcinoma. As for a series of anti-epithelial antibodies, Ber-EP4 or MOC-31 may be used in the demonstration of epithelial cell differentiation in cases where anti-cytokeratin’s do not produce a clearcut positivity or in cases where a false positivity for cytokeratin cannot be excluded, such as in sub mesothelial cells.

Immunogen:

Breast carcinoma cell line MCF-7

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

There are at least nine members of the paired box (PAX) gene family whose protein products are transcription factors involved in development. The conserved paired box DNA-binding domain is found in the N-terminal region of Pax proteins. An octomer and homeodomain sequence are conserved in the centre of the proteins. The Ser/Thr/ Pro-rich region in the Cterminal portion contains a conserved 100 amino acid transactivating domain. One of the best studied Pax family members, Pax-5 influences the expression of several B-cell-specific genes, such as CD 19 and CD 20. Pax-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells. Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. PAX-5 has been found to be important in both B cell and nervous system development.

Immunogen:

Human PAX-5 aa. 151-306

Presentation:

0.25 mg/ml in aqueous buffered solution containing BSA, glycerol, and ≤ 0.09% sodium azide

Specification:

This anti-Progesterone Receptor antibody reacts with progesterone receptor forms alpha and beta. This antibody stains nuclei in breast, ovarian and endometrial epithelia, as well as myometrial nuclei. Since the early 1990’s the immunohistochemical (IHC) assay determination of progesterone receptor status has replaced the dextran-coated charcoal method as a prognostic indicator in breast carcinoma. IHC has shown to be superior in prognostic significance when using any one of several available methods of quantitation using this technique.

Presentation:

Anti-Progesterone Receptor is a rabbit monoclonal from tissue culture supernatant diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide

Specification:

This antibody recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in several variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Immunogen:

Stimulated human leukocytes

Presentation:

Protein A/G purified antibody from Bioreactor Concentrate with 0.05% BSA and 0.05% Azide.

Specification:

Mammaglobin is a breast-associated glycoprotein distantly related to secretoglobin family that includes human uteroglobin and lipophilin. Unlike other secretoglobin family members, mammaglobin mRNA expression is breast specific, which has been shown to be a very sensitive marker of occult breast cancer cells in sentinel lymph nodes and peripheral blood. By paraffin immunohistochemistry, the overall sensitivity of mammaglobin for breast cancers was reported about 80%. When combined with other breast-restricted markers such as GCDFP-15, an overall sensitivity of 84% could be achieved. Mammaglobin can play a contributing role in the identification of primary sites of carcinomas presenting at metastatic sites. Positive control: normal human breast tissue. In normal breast tissue, 304-1A5 labels breast ductal and lobular epithelial cells. In tumor cells, it is reactive with all types of breast adenocarcinoma regardless tumor differentiation and types. Adenocarcinomas from other organs rarely express mammaglobin.

Presentation:

Purified antibody with 0.2% BSA and 15mM sodium azide