Name: Flow Cytometry Antibody CD10-APC-C750, Clone HI10a
- CD10-APC-C750TM is designed for flow cytometry (CF) use as a direct immunofluorescence reagent in the identification and enumeration of CD10 antigen-expressing cells. This reagent must be used by FC qualified personnel.
SUMMARY AND EXPLANATION
CYT-10AC750 mAb recognizes CD10 antigen, a 100 KDa glycoprotein belonging to a family of type II transmembrane metalloproteases. This molecule is expressed on immature T and B precursor cells but is lost as the cells reach maturation. However, it is re-expressed on proliferating B cells and mature neutrophils. CD10 antigen is a common acute lymphoblastic leukaemia associated antigen (CALLA) as it is found on lymphocytes from acute B-lymphoid leukaemia samples. It is also present on a wide variety of normal and neoplastic cell types including renal epithelium, fibroblasts, granulocytes, and some T-cell leukaemia, lymphoma, melanoma, and glioma cell lines. This antibody can therefore be used in the characterization of non-T (common) acute lymphoblastic leukemias and in the studies of early stages of hematopoietic differentiation. APC-C750TM is a tandem dye with a maximum emission peak at 779 nm, which grants bright signal, low unspecific noise and high photostability. When excited by light from a red laser, the APC fluorochrome can transfer energy to C750 molecule, which then emits at a longer wavelength. It is recommended to use a 780/60 nm longpass filter along with a red sensitive detector to use in conjunction antibodies conjugated with APC and APC-C750TM.
The purified monoclonal CD10 antibody conjugated with the tandem allophycocyanine-C750 (APC-C750TM) is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.
- Clone: HI10a.
- Isotype: IgG1.
- Amount per 0,15 mL vial: 50 tests (3 µL mAb per determination).
- Reagent is considered non-sterile.