Name: Flow Cytometry Antibody CD103-FITC Clone B-ly7
- Antibody CD103-FITC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of CD103 antigen-expressing cells.
CD103 is normally expressed at very high levels on intra-epithelial lymphocytes in non-lymphoid tissues, such as skin, lung, and gastrointestinal organs that express E-cadherin.
In contrast, CD103 is poorly expressed by T cells in blood, spleen, and lymph nodes. Since its discovery, CD103 has been widely used for hairy cell leukaemia (HCL) diagnosis and despite rare exceptions CD103 has been detected together with CD25 in virtually all HCL studies using flow cytometry. CD103 is not ordinarily detected in other types of Lymphoproliferative disorders except for rare cases. CD103 can also be used to define a specialized subset of CD8+ cytolytic T lymphocytes. This reagent must be used by flow cytometry qualified personal.
REAGENT COMPOSITION
The purified monoclonal CD103 antibody (mAb) conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing ≤0.1% sodium Azid.
- Clone: B-ly7.
- Isotype: IgG1.
- Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.
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