Name: Flow Cytometry Antibody CD185-APC, Clone MU5UBEE
- Antibody CD185-APC is a monoclonal antibody (mAb) labelled with allophycocyanin (APC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD185 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. CD185, also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a 7 transmembrane domain protein mainly expressed on B cells and CD4+ T cells (1, 2). CD185 has been selected by EuroFlow consortium in its B-CLPD (B-cell chronic lymphoproliferative diseases) panel.
The purified monoclonal CD185 antibody conjugated with allophycocyanin (APC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: MU5UBEE.
- Isotype: IgG2b.
- Amount per vial: 100 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.