Name: Flow Cytometry Antibody CD25-PE, Clone MEM-181
- Anti-human CD25 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD25 antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
CD25 antigen is part of interleukin-2 receptors. These receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2. The 55 kDa α chain is called CD25. The α chain alone, the βγ heterodimer, and the αβγ heterotrimer form the low-, intermediate- and high affinity IL-2R, respectively. The intermediate- and high affinity IL-2R play a role in intracellular signal transduction. CD25 is strongly expressed on the surface of T cells activated by antigens, mitogens (phytohemagglutinin A (PHA), concanavalin A (Con-A), pokeweed mitogen (PWM)), Staphylococcus aureus, viruses (HTLV-I, HTLV-II, Epstein-Barr virus), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide. In normal peripheral blood a variable staining of lymphoid cells (about 3-25%) is observed.
Purified anti-human CD25 mAb conjugated with different fluorochromes (see table above) is supplied in phosphate-buffered saline (PBS) containing 0,09% (m/v) sodium azide and 0.2% (w/v) bovine serum albumin (BSA).
- Clone: MEM-181.
- Isotype: Mouse/IgG1.
- Amount per vial: 1ml/ 100 tests (10 µl mAb per determination) (see table above).
- Purification: Affinity chromatography.
- Reagent is considered non-sterile.