Name: Flow Cytometry Antibody CD300e-APC, Clone UP-H2
- Antibody CD300e-APC is a monoclonal antibody (mAb) labelled with allophycocyanin (APC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD300e antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. IREM2, also known as CD300e, is a 34 kDa integral membrane glycoprotein which is a member of the CMFRF35 subfamily. IREM2 is expressed on monocytes and myeloid dendritic cell lineage. During normal monocytic development, CD300e is expressed in the last stages of maturation. CD300e has been selected by EuroFlow consortium in AML (acute myeloid leukemia) antibody panel.
The purified monoclonal CD300e antibody conjugated with allophycocyanin (APC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: UP-H2.
- Isotype: IgG1.
- Amount per vial: 100 tests (10ul/test).
- Reagent is considered non-sterile.