Name: Flow Cytometry Antibody CD81-APC-C750™, Clone M38
- Antibody Anti human CD81-APC-C750TM is a monoclonal antibody (mAb) labelled with the tandem allophycocyanine-C750 (APCC750TM) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD81 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personnel.
SUMMARY AND EXPLANATION
The CD81 (TAPA-1) antigen is expressed on normal lymphocytes, monocytes and eosinophils, but not on platelets and neutrophils. This antigen may be present in some cases as multimolecular complexes, in association with other members of the TM4 superfamily (CD37, CD53), or on the surface of B cells, in association with CD19 and/or CD21 and/or MHC class II antigens (3, 4). Most B lymphocytes, at all stages of cellular differentiation, express CD81 at relatively high levels. Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.
REAGENT COMPOSITION
Purified monoclonal CD81 antibody conjugated with the tandem allophycocyanine-C750 (APC-C750TM), supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.
- Clone: M38.
- Isotype: Mouse / IgG1.
- Amount per 0,15 ml vial: 50 tests (3 µL mAb per determination)
- Reagents are not considered sterile.
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