Name: Flow Cytometry Antibody cyCD79a-PE, Clone HM57
- Antibody cyCD79a-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD79a antigen by flow cytometry (FC). CD79a is expressed on B cells at various stages of differentiation, from pre-B cell to plasma cell stage. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). B lymphocytes are the producers of antibodies and mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. In normal cells CD79α expression is restricted to B cell lineage. cyCD79α is found in the cytoplasm of pro B- cells before Ig heavy-chain rearrangement and is extinguished in terminally differentiated plasma cells. CD79α is expressed in hairy cell leukaemia and in the majority of all low-grade B-cell leukaemias and lymphomas, such as lymphoplasmacytic, follicular, mantle cell, marginal zone and Burkitt’s lymphomas. Generally, the CD79α antigen is not found in acute myeloid leukaemia, but the FAB M3 class is a significant exception with more than 50% of cases displaying the antigen in the cytoplasm of malignant cells. Approximately 45% of T- cell ALL cases co-express CD3 and CD79α, and this co-expression phenotype may be clinically relevant.
Purified monoclonal cyCD79a Antibody conjugated with R-phycoerythrin (PE), supplied in phosphate buffered saline with 0,09% sodium azide.
- Clone: HM57.
- Isotype: Mouse / IgG1.
- Amount per 1 ml vial: 100 tests (10 ml/ test).
- Reagents are not considered sterile.