Name: Flow Cytometry Antibody IgA1-APC, Clone SAA1
IgA1-APC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgA1 expressing cells. IgA1-APC reacts with the Fc portion of the heavy chain of human IgA1.
In humans, 85–90% of IgA antibodies are found in body secretions such us saliva, tears, breast milk; gastrointestinal mucous membranes, urogenital and respiratory tracts and prostatic fluid, among others. The remaining fraction of 10–15% of IgA antibodies is found in blood, produced by B cells in the lymph nodes, bone marrow, and spleen. Two subclasses of human serum IgA exist. IgA1 tends to be monomeric and is present at a fivefold higher concentration than IgA2. Soluble IgA2 tends to be present more often in polymeric forms.
CYT-IGA1AP should be used with BulkLysisTM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low frequency subsets, as IgA1+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed. This reagent must be used by flow cytometry qualified personnel.
Purified monoclonal anti-IgA1 antibody (mAb) conjugated with allophycocyanine (APC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).
Isotype: Mouse IgG1.
Amount per vial: 25 tests using 3µL of mAb to 106 cells.