Name: Flow Cytometry Antibody IgD-FITC, Clone IADB6.
- Antibody IgD-FITC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgD expressing cells. IgD-FITC reacts with the delta chain of human IgD. IgD is barely detectable in the blood (0,001% of total serum Ig) and is rarely secreted. It is observed only on the surface of immature, peripheral B cells that already express IgM. The expression of IgD is transient; upon activation of the B cell by the binding of antigen, the surface expression of mIgD, but not mIgM, is abrogated.
CYT-IGDF should be used with BulkLysisTM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low-frequency subsets, as IgD+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.
Purified monoclonal anti-IgD antibody (mAb) conjugated with fluorescein isothiocyante (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).
- Clone: IADB6
- Isotype: Mouse IgG2a.
- Amount per vial: 25 tests using 3µL of mAb to 106 cells.