Name: AMACR/p504s (Clones13H4+4A4)
Description and applications: The main use of the combination of both antibodies in a single reagent is simultaneously immunostain prostate glands in order to detect whether or not neoplastic. In the case of prostate adenocarcinoma, neoplastic glands must present cytoplasmic staining produced by expression of p504s and absence of nuclear staining as these glands lack basal cells and thus are negative for p63. Normal or hyperplastic glands must present nuclear staining in the basal cell layer. The
Due to the different pattern of staining of the antibodies included in the cocktail, the detection could be realised with a polyvalent HRP detection systems.
Nevertheless, as the antibodies are developed in two different host, a dual detection system can be used.
Composition: anti-human AMACR/p50s rabbit monoclonal antibody + anti-human p63 mouse monoclonal antibody prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Immunogen: Human AMACR polypeptide + aminoacids 1-250 of the N-terminal portion of the human p63 protein