آنتی بادی مونوکلونال فلوسایتومتری ™Kappa-APC-C750 ،کلون Polyclonal

Name: Flow Cytometry Antibody Kappa-APC-C750™, Clone Polyclonal

• Antibody Kappa-APC-C750^TM is a polyclonal antibody (pAb) labelled with the tandem allophycocyanine-C750 (APC-C750^TM) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells that express human Kappa immunoglobulin Light Chains by flow cytometry (FC). This reagent must be used by FC qualified personnel.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and Natural Killer cells (NK). B lymphocytes are the producers of antibodies and mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses.  Most B cells, with the exception of pre-B progenitors, pre B-cells, and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type (Kappa or Lambda). In normal peripheral blood and lymph nodes, there is a mixture of Kappa positive and Lambda positive cells, with two-thirds of the cells expressing Kappa and one-third expressing Lambda. Since lymphoid neoplasms are usually clonal expansions of a single cell, malignant cells uniformly express the same Light Chain isotype. Neoplastic B cell lymphoprolipherative disorders can frequently be suspected based on the demonstration of a marker predominance of cells expressing a single Light Chain type. The Lambda immunoglobulin Light Chain count is generally expressed as a percentage of B cells present in the sample which can be determined by FC based on its positive expression of CD19.

APC-C750TM is a tandem dye with a maximum emission peak at 779 nm, which grants bright signal, low unspecific noise and high photostability. When excited by light from a red laser, the APC fluorochrome can transfer energy to C750 molecule, which then emits at a longer wavelength. It is recommended to use a 780/60 nm longpass filter along with a red sensitive detector to use in conjunction antibodies conjugated with APC and APC-C750^TM.

REAGENT COMPOSITION

Purified polyclonal antibody anti-human Kappa Light Chains, Goat F(ab’)2, conjugated with tandem allophycocyanineC750 (APC-C750^TM), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and 0,2% (w/v) bovine serum albumin (BSA).

• Clone: Polyclonal
• Isotype: Goat / Polyclonal
• Amount per vial: 50 tests (3µL/Test).

Reagents are not considered sterile