Name: Flow Cytometry Antibody TCRgd-PECyanine7, Clone 11F2
- Antibody TCRgd-PECyanine7 is a monoclonal antibody (mAb) labelled with the tandem R-Phycoerythrin-Cyanine 7 (PECy7), designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of T cells which express the TCRgd. This reagent must be used by FC qualified personal.
SUMMARY AND EXPLANATION
The g/d TCR is a heterodimeric glycoprotein that is noncovalently associated with the CD3 antigen complex. The g and d TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The g chain forms either disulfide-linked or non-disulfide-linked heterodimers with the d -subunit. TCR g/d is present on a minor subset of T lymphocytes in peripheral blood, thymus, spleen, and lymph node. TCR g/d -positive T lymphocytes comprise 1% to 9% of normal peripheral blood lymphocytes and less than 2% of normal thymocytes.
REAGENT COMPOSITION
Purified TCRgd antibody conjugated with PECyanine7, is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.
- Clone: 11F2
- Isotype: Mouse / IgG1
- Amount per 0,3mL vial: 100 tests (3 µL mAb to 106 cells).
- Reagents are not considered sterile
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