Name: Keratin CAM5.2 Antibody Clone CAM5.2
Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins. Keratin CAM 5.2 antibody reacts with 38-50 kD molecular weight keratins present in most simple and ductal epithelia. This antibody is useful for identifying normal and neoplastic epithelial cells in histological sections. Positive stain is obtained in adenocarcinomas of the breast, bowel, liver, renal, neuroendocrine carcinomas (eg Merkel cell tumor), mesotheliomas , germ cell tumors (except seminoma), synovial and epithelioid sarcoma. Some tissues of non-epithelial origin can be immunostained as smooth muscle, some breast sarcomas, meningiomas and malignant neuroblastomas. CAM 5.2 doesn´t stain the stratified epitheliums and does not react with carcinomas arising in the esophagus, vagina and cervix. Negative results were also obtained in lymphomas, melanomas, meningiomas, sarcomas and seminomas.
Composition: IgG2a immunoglobulin, obtained from mouse ascites purified by chromatography and diluted in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.
Imunogen: human colorectal carcinoma cell line HT24.