آنتی‌ بادی MDM2 (IF2)

Name: Mouse anti-human MDM2 Monoclonal Antibody clone IF2

Description and aplications: MDM2 (murine double minute 2) or E3 ubiquitinprotein ligase, is a protein encoded by the chromosomal region 12q13-15, and mediates the ubiquitination of p53 / TP53 leading to its degradation by the proteasome. Due to MDM2 binding to the transcriptional activation domain of p53 / p73 TP53- and p73/ TP73- inhibits G1 arrest of the cell cycle and apoptosis. It also acts as an E3 ubiquitin ligase and ARRB1 itself. Allows nuclear export of p53 / TP53. It promotes ubiquitin-dependent proteasomeindependent degradation of the retinoblastoma protein (RB1). This monoclonal antibody reacts specifically with human MDM2 protein. Immunohistochemical detection of MDM2 and CDK4 overexpression may be helpful to distinguish between well-differentiated liposarcomas and benign tumors of adipocytes (sensitivity / specificity 100% / 58.8% and 68.4% / 88.2%, respectively) and between dedifferentiated liposarcoma from other malignant mesenchymal tumors. MDM2 and CDK4 are expressed in 100% and 93% of dedifferentiated liposarcoma and in only 18% of malignant tumors of peripheral nerve sheaths, mixofibrosarcomas, embryonal rhabdomyosarcoma and 1-6% of poorly differentiated sarcomas (malignant fibrous histiocytoma and leiomyosarcoma). Overexpression of MDM2 / CDK4 proteins is also a highly sensitive marker for the diagnosis of low grade osteosarcomas.

Composition: anti-human MDM2 mouse monoclonal antibody purified from ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: Synthetic peptide derived from the Nterminal region of human MDM2