Name: Flow Cytometry Antibody CD2-FITC, Clone RPA-2.10

• Antibody CD2-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD2 antigen by flow cytometry (FC). CD2 can be considered a pan-T cell antigen, as it is expressed on the surface of all normal Tlymphocytes. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

 The CYT-2F7 mAb recognizes the 50kDa lymphocyte surface antigen expressed on all peripheral blood T lymphocytes, the majority of thymocytes and malignant cells of T cell origin, including T ALL cells. CD2 is a useful marker in the assessment of lymphoid malignancies as it is expressed in the majority of precursor and posthymic lymphomas and leukemias. In some neoplastic T-cell populations e.g. in peripheral T cell lymphomas, CD2 may be aberrantly deleted.

 REAGENT COMPOSITION

Purified monoclonal CD2 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide.

• Clone: RPA-2.10
• Isotype: IgG1
• Purification: Affinity chromatography
• Amount per 1 mL vial: 200 tests (5 µL mAb to 106 cells)
• Reagents are not considered sterile.

Name: CD3-FITC, (Clone 33-2A3 (Cris-7))

 Antibody CD3-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the human CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

The CYT-3F8 mAb recognizes the CD3 antigen present in T cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of posttransplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. The T lymphocyte (CD3+) count is generally expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by flow cytometry based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

Purified monoclonal CD3 Antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.

• Clone: 33-2A3 (Cris-7).
• Isotype: IgG2a.
• Purification: Affinity chromatography.
• Size: 200 tests (1 ml per vial)
• Usage: 5 µl mAb per determination
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD2-FITC, Clone RPA-2.10

• Antibody CD2-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD2 antigen by flow cytometry (FC). CD2 can be considered a pan-T cell antigen, as it is expressed on the surface of all normal Tlymphocytes. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

 The CYT-2F7 mAb recognizes the 50kDa lymphocyte surface antigen expressed on all peripheral blood T lymphocytes, the majority of thymocytes and malignant cells of T cell origin, including T ALL cells. CD2 is a useful marker in the assessment of lymphoid malignancies as it is expressed in the majority of precursor and posthymic lymphomas and leukemias. In some neoplastic T-cell populations e.g. in peripheral T cell lymphomas, CD2 may be aberrantly deleted.

 REAGENT COMPOSITION

Purified monoclonal CD2 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide.

• Clone: RPA-2.10
• Isotype: IgG1
• Purification: Affinity chromatography
• Amount per 1 mL vial: 200 tests (5 µL mAb to 106 cells)
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD4-APC, Clone HP2\6

Ø  Antibody CD4-APC is a monoclonal antibody (mAb) labelled with the Allophycocyanine (APC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD4 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. The CYT-4AP3 MAb recognizes the CD4 antigen present in helper/inducer T cells (CD3+CD4+) and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. Individuals with HIV typically exhibit a steady decrease of helper/inducer T lymphocyte counts as the infection progresses. The CD4 antigen is also detected on some monocytes.

REAGENT COMPOSITION

Purified monoclonal CD4 Antibody Allophycocyanine (APC) conjugated, supplied in phosphate buffered saline with 0,09% sodium azide.

• Clone: HP2/6

• Isotype: IgG2a

• Purification: Affinity chromatography

• Amount per 1 ml vial: 200 tests (5 µL mAb to 106 cells)

• Reagents are not considered sterile.

Name: CD4-PerCP-Cyanine5.5, Clone RPA-T4

• Antibody CD4 is a monoclonal antibody (mAb) labelled with different fluorochromes (see table), designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD4 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. CD4 mAb recognizes the CD4 antigen present in helper/inducer T cells (CD3+CD4+) and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. Individuals with HIV typically exhibit a steady decrease of helper/inducer T lymphocyte counts as the infection progresses. The CD4 antigen is also detected on some monocytes.

REAGENT COMPOSITION

Purified monoclonal CD4 antibody conjugated with different fluorochromes (see table) is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0,09% (m/v) sodium azide.

• Purification: Affinity chromatography
• Amount per vial: 50, 100 or 200 tests (3 or 5 µl mAb to 106 cells) (see table).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD5-FITC, Clone MCD5

• Antibody CD5-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD5 antigen by flow cytometry (FC). CD5 is a useful marker for the identification of certain lymphoprolipherative syndromes when is used within an extended immunophenotyping panel. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

The CYT-5F4 mAb recognizes a 67 kDa antigen expressed by a majority of thymocytes and mature T cells and a subset of B cells. CD5 is expressed at low density on thymocytes and at high density on all mature T lymphocytes. The majority of T cell malignancies expresses CD5, and almost 85% of T cell acute lymphoblastic leukemias are CD5 positive. Further, CD5 is expressed at low density on a small subset of mature B lymphocytes, which is expanded in several autoimmune disorders as well as in some B-cell derived lymphoproliferative disorders such as chronic lymphocytic leukemia (CLL) and centrocytic leukemia.

REAGENT COMPOSITION

Purified monoclonal CD5 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide.

• Clone: MCD5
• Isotype: IgG2b
• Purification: Affinity chromatography
• Amount per 1 ml vial: 200 tests (5 ml mAb to 106 cells)
• Reagents are not considered sterile