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INTRODUCTION: Rearrangements and abnormal expression of the FOXO1 gene (also known as FKH1, FKHR or FOXO1A) have been observed in the alveolar rhabdomyosarcoma, prostate carcinoma and other types of tumors. In the case of alveolar rhabdomyosarcoma, fragments of the 3’ region of the FOXO1 gene translocate with fragments of the 5’ portions of the PAX3 and PAX7 genes, located in the chromosome 2 and 1 respectively.  Those cases showing the PAX3-FOXO1 fusion in metastases present a worse prognosis, while in primary tumors, the development of the disease is independent of the type of translocation. In the prostate cancer, deletions of the FOXO1 gene have been detected in up to 30% of the cases.

INTENDED USE:  The FOXO1 Break Apart FISH probe is designed to detect rearrangements in the FOXO1 human gene located in the band of the chromosome 13q14.11. Aside from detecting breaks that can lead to translocation of parts of the gene, to its inversion or to its fusion with other genes, the probe can also be used to identify other aberrations of the FOXO1 gene such as deletions or amplifications.

PROBE DESCRIPTION: The 5’ telomeric fragment of the FOXO1 Break Apart FISH probe labeled with the fluorochrome CytoOrange covers the center and the 5’ portion (beginning) of the FOXO1 gene and some adjacent genomic sequences. The 3’ centromeric fragment of the FOXO1 Break apart FISH probe labeled with CytoGreen covers the 3’ part (end) of the FOXO1 gene as well as the adjacent and inferior sequences of the gene. Both probes are flanking sequences of the FOXO1 gene, in which variable cut-off points have been observed.

INTRODUCTION: Rearrangements and abnormal expression of the TFE3 gene (also known as TFEA, RCCP2, RCCX1 or bHLHe33) have been described in a special subtype of renal cell carcinoma associated with Xp11.2 translocation, in alveolar sarcoma and other tumor types.

INTENDED USE: The TFE3 Break Apart FISH probe is designed to detect rearrangements in the TFE3 human gene located in the band of the chromosome Xp11.23. Aside from detecting breaks that can lead to translocation of parts of the gene, to its inversion or to its fusion with other genes, the probe can also be used to identify other aberrations of TFE3 such as deletions or amplifications.

PROBE DESCRIPTION: The 5’ centromeric fragment of the TFE3 Break Apart FISH probe labeled with the fluorochrome CytoOrange covers the 5’ portion (beginning) of the TFE3 gene and some adjacent genomic sequences. The 3’ telomeric fragment of the TFE3 Break apart FISH probe labeled with CytoGreen covers the 3’ part (end) and the adjacent and inferior sequences of the gene. Both probes are flanking sequences in the TFE3 gene, in which variable cut-off points have been observed.

INTRODUCTION:  Mixoid / round cell liposarcoma (MRCLS) is the most common liposarcoma. In comparison with other liposarcomas, MRCLS has a greater tendency to metastasize. Rearrangements and abnormal expression of the DDIT3 gene also known as CHOP, CEBPZ, CHOP10, CHOP-10, GADD153 in myxoid liposarcoma and other malignancies have been observed. One most common abnormality is the reciprocal translocation t (12; 16) (q13; p11) that is present in up to 95% of cases. The resulting FUS-DDIT3 fusion protein has oncogenic potential and interferes with adipogenic differentiation. EWSR1 is an alternative translocation partner of DDIT3 and the resulting fusion protein EWSR1-DDIT3, which originates at t (12; 22) (q13; q12), accounts for <5% of MRCLS cases. The DDIT3 rearrangements are highly specific for MRCLS due to the presence of the FUS-DDIT3 or EWSR1-DDIT3 fusion genes.

INTENDED USE: The DDIT3 Break Apart FISH probe is designed to detect rearrangements in the human DDIT3 gene located in the band of chromosome 12q13.3. In addition to detecting breaks that can lead to the translocation of parts of the gene, its inversion or its fusion to other genes, the probe can also be used to identify other aberrations of the DDIT3 gene such as deletions or amplifications.

PROBE DESCRIPTION: The telomeric 5 ‘fragment of the DDIT3 Break Apart FISH probe labeled with the CytoGreen fluorochrome covers some genomic sequences adjacent to the 5′ end of the DDIT3 geneThe 3’-centromeric fragment of the DDIT3 Break Apart FISH probe labeled with CytoOrange covers some genomic sequences of the 3 ‘end of the gene.The two probes are sequences that flank the DDIT3 gene region where several cut points have been observed.

INTRODUCTION: Abnormalities in ATM – also known as AT1, ATA, ATC, ATD, ATE, ATDC, TEL1 or TELO1 – occur in ataxia telangiectasia, and have also been observed in chronic lymphocytic leukemia (CLL) and other malignancies. The GLI1 gene – also called GLI – is an oncogene that is expressed and sometimes amplified in several human cancers.

INTENDED USE: The ATM/GLI1 FISH Probe Kit is designed to detect rearrangements involving the human ATM and GLI1 genes located on chromosome bands 11q22.3 and 12q13.3, respectively.

PROBE DESCRIPTION:The ATM Probe of the ATM/GLI FISH Probe kit labeled with the fluorochrome CytoOrange covers a chromosomal region which includes the entire ATM gene. The GLI Probe of the ATM/GLI FISH Probe Kit labeled with CytoGreen covers a chromosomal region which includes the entire GLI1 gene. Both probes are designed to measure the copy number of the human ATM and GLI1 gene located on chromosome band 11q22.3 and 12q13.3, respectively.

INTRODUCTION: The product of the human BCR gene, breakpoint cluster region (BCR) protein, also called BCR1, PHL or renal carcinoma antigen NY-REN-26, is a ubiquitously expressed serine/threonine kinase and GTPase-activating cytoplasmic enzyme. ABL1 (Abelson murine leukemia viral oncogene homolog 1, also called c-ABL, JTK7, p150) is a cytoplasmic and nuclear tyrosine kinase involved in a variety of cellular processes including migration, adhesion, differentiation and apoptosis. It is also an important component of the T-cell receptor signaling system and required for T-cell development and function.
The t(9;22)(q34;q12) reciprocal translocation between BCR and ABL1 leads to a lengthened chromosome 9 (9q+) and a shortened chromosome 22 (der(22), 22-), also called Philadelphia-chromosome (Ph, Ph1). Discovered in 1960, it is a hallmark of chronic myelogenous leukemia (CML) and was the first consistent genetic abnormality identified in any human cancer.The fusion combines varying portions of the 5’- part of BCR with the 3’-part (exons 2-11) of ABL1 and leads to the production of a fusion protein in which the tyrosine kinase of ABL is constitutively activated.The Philadelphia chromosome is present in virtually all CML cases.16 However, it is also found in a subset of adult and pediatric acute lymphoblastic leukemia (ALL) and sometimes in acute myelogenous leukemia (AML) and other malignancies such as the very rare chronic neutrophilic leukemia (CNL).
INTENDED USE: The BCR-ABL1 Fusion/Translocation FISH probe kit pair is designed to detect rearrangements involving regions of the human BCR (Breakpoint cluster region) locus, located on chromosome band 22q11.23, and of the human ABL1 gene on 9q34.12. The Probe kit is optimized to reveal the typical t(9;22)(q34;q11) translocation as well as other translocations between the two genes, in metaphase and interphase blood or bone marrow cells.

PROBE DESCRIPTION:
The BCR Probe of the BCR/ABL1 Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoGreen covers the entire BCR gene along with some upstream (5’) and downstream (3’) flanking untranslated genomic sequences. The probe overlaps the known major and minor breakpoints observed in the region. The ABL1 Probe of the BCR/ABL1 Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoOrange covers the complete ABL1 gene on 9q34.12, including the common breakpoint region upstream of exon 2, as well as adjacent 5’ and 3’ untranslated genomic sequences.

INTRODUCTION:The human CBFB genes encode a protein named Core-binding factor subunit beta.
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2).Rearrangements and abnormal expression of the CBFB gene – also known as CBFb or PEBP2B – have been observed in acute myeloid leukemia (AML) and other hematological malignancies.
INTENDED USE: The CBFB Break Apart FISH probe Kit t is designed to detect rearrangements in the human CBFB gene located on chromosome band 16q22.1. In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes, the probe set can also be used to identify other CBFB aberrations such as deletions or amplifications.

PROBE DESCRIPTION:The 5’ centromeric fragment of the CBFB Break Apart FISH Probe kit labeled with the fluorochrome CytoOrange covers the 5’ (start) portion of the CBFB gene and some adjacent genomic sequences. The 3’ Telomeric fragment of the CBFB Break apart FISH Probe Kit labeled with CytoGreen covers the 3’ (end) part as well as sequences downstream of the gene. The two probes are flanking sequences across the CBFB gene in which variable breakpoints have been observed.

INTRODUCTION: Expression of the CUX1 gene – also known as CDP, CUX, p75, CASP, CDP1, COY1, Clox, p100, p110, p200, CUTL1, GOLIM6, CDP/Cut, Cux/CDP or Nbla10317 – has been observed elevated in pancreatic, breast and other cancers. Duplications and other anomalies in the region of the VIPR2 gene – also called VPAC2, VPAC2R, VIP-R-2, VPCAP2R, PACAP R3, DUP7q36.3, PACAP-R-3 or C16DUPq36.3 – are associated with schizophrenia, prenatal malformations and some intestinal malignancies.
INTENDED USE: The CUX1/VIPR2/CCP7 FISH Probe Kit is designed to detect the human CUX1 gene located on chromosome band 7q22.1, and the VIPR2 gene on chromosome band 7q36.3, along with the number of chromosome 7 copies per cell.

PROBE DESCRIPTION: The VIPR2 Probe of the CUX1/VIPR2/CCP7 FISH Probe Kit labeled with the fluorochrome CytoOrange covers a chromosomal region which includes the entire CUX1 gene. The CUX1 Probe of the CUX1/VIPR2/CCP7 FISH Probe Kit labeled with CytoGreen covers a chromosomal region which includes the entire VIPR2 gene. The CCP7 Probe of the CUX1/VIPR2/CCP7 FISH Probe Kit labeled with the fluorochrome CytoAqua is derived from chromosome 7-specific alpha satellite DNA, is designed to serve as a control to determine the number of chromosome 7 copies per cell.
The probes are designed measure the copy number of the human CUX1 and VIPR2 genes located on chromosome bands 7q22.1 and 7q36.3, respectively.

INTRODUCTION: The DLEU1 gene was originally identified as a potential tumor suppressor gene. The TP53 gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Rearrangements and abnormal expression of the DLEU1 gene region – also known as BCMS, DLB1, LEU1, LEU2, XTP6, BCMS1, DLEU2, LINC00021 or NCRNA00021 – and the TP53 gene – also known as P53, BCC7, LFS1 or TRP53– have been observed in B-cell chronic lymphocytic leukemia (CLL) and other malignancies.
INTENDED USE: The DLEU1/TP53 FISH Probe Kit is designed to detect the human DLEU1 and TP53 genes located on chromosome bands 13q14.2 and 17p13.1, respectively. Both probes are designed to measure the copy number of the human DLEU1 and TP53 genes located on chromosome bands 13q14.2 and 17p13.1, respectively.

PROBE DESCRIPTION: The DLEU1 Probe of the DLEU1/TP53 FISH Probe Kit labeled with the fluorochrome CytoOrange covers the center sequences and the 5′ (start) portion of the DLEU1 gene and some adjacent genomic sequences. The TP53 Probe of the DLEU1/TP53 FISH Probe Kit labeled with CytoGreen covers a chromosomal region which includes the entire TP53 gene.

INTRODUCTION:The DLEU1 gene was originally identified as a potential tumor suppressor gene.The ZBTB16 gene is a member of the Kruppel C2H2 type zinc finger protein family and encodes a transcription factor containing nine Kruppel-type zinc finger domains at the C-terminus. The ZBTB16 protein is located in the cell nucleus and is involved in the progression of the cell cycle, interacting with histone deacetylases. Rearrangements and abnormal expression of the DLEU1 gene region – also known as BCMS, DLB1, LEU1, LEU2, XTP6, BCMS1, DLEU2, LINC00021 or NCRNA00021 – have been observed in B-cell chronic lymphocytic leukemia (CLL), multiple myeloma (MM) and other malignancies. Fusions and aberrant expression of the ZBTB16 gene – also known as PLZF or ZNF145 – have been reported in acute promyelocytic leukemia and other neoplasias.
INTENDED USE: The DLEU1/ZBTB16 FISH Probe Kit is designed to detect the human DLEU1 gene, located on chromosome band 13q14.2, and the ZBTB16 gene on chromosome band 11q23.2.

PROBE DESCRIPTION:The DLEU1 Probe of the DLEU1/ZBTB16 FISH Probe kit labeled with the fluorochrome CytoOrange covers a chromosomal region which includes the majority of the DLEU1 gene and genomic sequences adjacent to the 5′ end of the gene. The ZBTB16 Probe of the DLEU1/ZBTB16 FISH Probe Kit labeled with CytoGreen e covers a chromosomal region which includes the entire ZBTB16 gene. Both probes are designed to measure the copy number of the human DLEU1 and ZBTB16 genes located on chromosome bands 13q14.2 and 11q23.2, respectively.

INTRODUCTION:PML gene encodes in humans a protein calledPromyelocytic leukemia protein (PML) (also known as MYL, RNF71, PP8675 or TRIM19). PML protein is a tumor suppressor protein required for the assembly of a number of nuclear structures, called PML-nuclear bodies, which form amongst the chromatinof the cell nucleus. The RARA gene encodes in humans a nuclear receptor protein called Retinoic acid receptor alpha (RAR-α), also known as NR1B1 (nuclear receptor subfamily 1, group B, member 1). Rearrangements between the two genes have been observed in acute promyelocytic leukemia and other malignancies.
INTENDED USE: The PML-RARA Fusion/Translocation FISH Probe Kit is designed to detect rearrangements involving the human PML and RARA genes, located on chromosome bands 15q24.1 and 17q21.2, respectively.

PROBE DESCRIPTION: The RARA Probe of the PML-RARA Fusion/Translocation FISH Probe labeled with the fluorochrome CytoGreen overs a chromosomal region including the entire RARA gene and some sequences upstream (5′ start) and downstream (3′ end) of the gene. The PML Probe of the PML-RARA
Fusion/Translocation FISH Probe labeled with the fluorochrome CytoOrange covers a chromosomal region which includes the entire PML gene and some
sequences around the gene.

INTRODUCTION: MAF gene encode in humanas the Transcription factor Maf also known as proto-oncogene c-Maf or V-maf musculoaponeurotic fibrosarcoma oncogene homolog. t(14;16)(q32;q23) represents 14q32/IGH rearrangement and belongs to the group of IGH/MAF translocations (rearrangements of the genes from the MAF oncogene family MAF, MAFA and MAFB with the IGH locus). As the other two IGH/MAF translocations t(8;14)q24;q32) andt(14;20)(q13;32) is described only in plasma cell neoplasms (PCN). t(14;16)(q32;q23) resulted in the juxtaposition of the oncogene MAF ( located at 16q23) to the strong enhancer of the IGH gene (located at 14q32) causing its up regulation in the plasma cells. This anomaly is found in both multiple myeloma (MM) and its precursor monoclonal gammapathy of undetermined significance (MGUS) respectively in 5% and 1-5% of the cases with 14q32 rearrangements.
INTENDED USE: The IGH-MAF Fusion/Translocation FISH probe kit is designed to detect rearrangements involving regions of the human IGH locus, located on chromosome band 14q32.33, and of the human MAF gene on 16q23.2.

PROBE DESCRIPTION: The IGH Probe of the IGH-MAF Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoGreen covers the 5’ and the center sequences of the IGH locus, and it also covers the 3’ (end) part and the neighboring downstream region. The MAF Probe of the IGH-MAF Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoOrange covers the chromosomal region that includes the entire MAF gene.

INTRODUCTION: Rearrangements and abnormal expression of the MYC gene (also known as MRTL, MYCC, c-Myc or bHLHe39) have been described in Burkitt’s lymphoma, where its positivity has diagnostic utility within a clinical, morphological and immunohistochemical context. On the other hand, in large or non-classifiable diffuse B-cell lymphoma, the MYC translocation has a prognostic value and dictates a more aggressive treatment of the neoplasm. On the other hand, together with the determination of the translocation of the BCL2 and BCL6 genes, it aims to establish the double or triple-hit character of the neoplasm. Other hematological neoplasms, myelomas, as well as breast, cervical, colon, ovarian and other tumors may present MYC translocation.
Most Burkitt lymphoma cases show the t(8;14)(q24;q32) [MYC-IGH] and less commonly the t(8;22)(q24;q11) or t(2;8)(p12;q24). The translocation is present in both the endemic African Burkitt lymphoma and in the non-endemic tumor type (Europe, America, and Japan). In case the Burkitt lymphoma infiltrated the bone marrow (leukemic phase) the MYC-translocation can be demonstrated in the bone marrow or blood as well.
INTENDED USE: The IGH-MYC Fusion/Translocation FISH probe kit t is designed to detect rearrangements involving the human IGH locus and MYC gene, located on chromosome bands 14q32.33 and 8q24.21, respectively.

PROBE DESCRIPTION: The IGH Probe of the IGH-MYC Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoGreen covers the 5’ and the center sequences of the IGH locus, and it also covers the 3’ (end) part and the neighboring downstream region. The MYC Probe of the IGH-MYC Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoOrange covers some genomic sequences downstream of the 5’ (start) portion of the MYC gene, and it also covers the sequences adjacent to the 3’ end of the gene. The probe set is optimized to reveal translocations between the two regions.