INTRODUCTION:The CSF1R gene (known also as c-FMS) is a receptor for a cytokine called colony stimulating factor 1 that in humans encode cell-surface protein called Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115 (Cluster of Differentiation 115). he encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. The EGR1 gene encode in humans a protein called EGR-1 (Early growth response protein 1) also known as Zif268 (zinc finger protein 225) or NGFI-A (nerve growth factor-induced protein A). EGR-1 is a transcription factor. Abnormalities in CSF1R – also known as FMS, CSFR, FIM2, HDLS, CFMS, CD115, CSF1R or M-CSF-R – and abnormalities in EGR1 – also Known as ERBB, HER1, mENA, ERBB1, PIG61 or NISBD2 – have been observed in myeloid malignancies, lung cancer and several other cancer types.
INTENDED USE: The CSF1R/EGR1 FISH Probe Kit is designed to detect the human CSF1R gene, located on chromosome band 5q32, and the EGR1 gene on chromosome band 5q31.2.
PROBE DESCRIPTION:The CSF1R Probe of the CSF1R/EGR1 FISH Probe kit labeled with the fluorochrome CytoOrange covers a chromosomal region which includes the entire CSF1R gene. The EGR1 Probe of the CSF1R/EGR1 FISH Probe Kit labeled with CytoGreen covers a chromosomal region which includes the entire EGR1 gene. Both probes are designed to measure the copy number of the human CSF1R and EGR1 gene located on chromosome band 5q32 and 5q31.2, respectively.