INTRODUCTION:The human CBFB genes encode a protein named Core-binding factor subunit beta.
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2).Rearrangements and abnormal expression of the CBFB gene – also known as CBFb or PEBP2B – have been observed in acute myeloid leukemia (AML) and other hematological malignancies.
INTENDED USE: The CBFB Break Apart FISH probe Kit t is designed to detect rearrangements in the human CBFB gene located on chromosome band 16q22.1. In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes, the probe set can also be used to identify other CBFB aberrations such as deletions or amplifications.
PROBE DESCRIPTION:The 5’ centromeric fragment of the CBFB Break Apart FISH Probe kit labeled with the fluorochrome CytoOrange covers the 5’ (start) portion of the CBFB gene and some adjacent genomic sequences. The 3’ Telomeric fragment of the CBFB Break apart FISH Probe Kit labeled with CytoGreen covers the 3’ (end) part as well as sequences downstream of the gene. The two probes are flanking sequences across the CBFB gene in which variable breakpoints have been observed.