INTRODUCTION: Rearrangements and abnormal expression of the MALT1 gene (also known as MLT, MLT1 or IMD12), in B-cell lymphomas and other malignant diseases have been described. There are two types of translocations that involve the MALT1 gene described in lymphomas: t(11;18)(q21;q21.32) that involves BIRC3/MALT1 (the most frequent) and t(14;18)(q32;q21.32) of the IGH/MALT1 genes. Up to 33% of the mucosa-associated lymphoid tissue B-cell lymphoma (MALT-type B-cell lymphoma) present translocation, whereas the vast majority of primary MALT lymphomas in lymph node or spleen are negative.
INTENDED USE: The MALT1 Break Apart FISH probe is designed to detect rearrangements in the MALT1 human gene located in the band of the chromosome 18q21.32. Aside from detecting breaks that can lead to translocation of parts of the gene, to its inversion or to its fusion with other genes, the probe can also be used to identify other aberrations of MALT1 such as deletions or amplifications.
PROBE DESCRIPTION: The 5’ centromeric fragment of the MALT1 Break Apart FISH probe labeled with the fluorochrome CytoOrange covers the 5’ portion (beginning) of the MALT1 gene and some adjacent genomic sequences. The 3’ telomeric fragment of the MALT1 Break apart FISH probe labeled with CytoGreen covers the 3’ part (end) and the adjacent and inferior sequences of the gene. Both probes are flanking sequences of the MALT1 gene, in which variable cut-off points have been observed.