INTRODUCTION: The rearrangements and the abnormal expression of the MET gene (also known as HGFR, AUTS9, RCCP2 or c-Met) have been described in hereditary and sporadic renal tumors and other types of tumors. The amplification of the MET gene has been detected in lung, breast, gastrointestinal and prostate carcinomas as well as in gliomas and melanomas, among others. In these tumors, the amplification of the gene is a negative prognostic factor and, in some cases, it is associated with resistance to the treatment.
INTENDED USE: The MET/CEN7 FISH probe is designed to detect amplifications of the MET human gene located in the band of the chromosome 7q31.2, in relation to the centromere of the Chromosome 7.
PROBE DESCRIPTION: The MET FISH probe labeled with the CytoOrange fluorochrome covers a chromosome region that includes the whole MET gene. The CEN7 FISH probe labeled with the CytoGreen fluorochrome is derived from the alpha satellite region of the centromere of the chromosome 7 and is designed as a control to determine the number of copies of the chromosome 7 per cell.