INTRODUCTION: An abnormal expression of the MDM2 gene (also known as MDM2, hdm2 or ACTFS) has been described, generally associated by amplification of the same with a 7% of the human neoplastic processes. The neoplasms that more frequently show MDM2 gene amplification are atypical lipomatous tumors/well-differentiated liposarcomas, undifferentiated liposarcomas, intimal sarcomas and low-grade osteosarcomas (parosteal/intramedullary).
INTENDED USE: The MDM2/CEN12 FISH probe is designed to analyze the MDM2 human gene located in the band of the chromosome 12q15, in relation to the centromere of the Chromosome 12.
PROBE DESCRIPTION: The MDM2 FISH Probe labeled with the CytoOrange fluorochrome covers a chromosome region that includes the whole MDM2 gene. The CCP12 FISH Probe labeled with the CytoGreen fluorochrome is derived from the alpha satellite region of the centromere of the chromosome 12 and is designed as a control to determine the number of copies per cell of the chromosome 12.