INTRODUCTION: Rearrangements and abnormal expression of the IRF4 gene – also known as NF-EM5, MUM1, LSIRF or IRF-4 – and the DUSP22 gene – also called JKAP, JSP-1, JSP1, LMW-DSP2, LMWDSP2, MKP-x, MKPX or VHX – have been observed in multiple myeloma (MM) and other lymphoid malignancies, viral malignancies, skin cancer and lymphomatoid papulosis (LyP), a chronic papulonecrotic or papulonodular skin disease with histologic features suggestive of a malignant lymphoma.
INTENDED USE: The IRF4/DUSP22 Break Apart FISH probe Kit is designed to detect rearrangements in the human IRF4 and DUSP22 genes and the surrounding regions located on chromosome band 6p25.3. In addition to revealing breaks, which can lead to translocation of parts of the genes, inversion, or their fusion to other genes, the probe set can also be used to identify other IRF4 and DUSP22 aberrations such as deletions or amplifications.
PROBE DESCRIPTION:The 5’ telomeric fragment of the IRF4/DUSP22 Break Apart FISH Probe kit labeled with the fluorochrome CytoGreen covers the entire DUSP22 gene, genomic sequences adjacent to the 5’ (start) and the 3′ (end) portions of the gene, and genomic sequences upstream of the IRF4 gene’s 5′ (start) end. The 3’ centromeric fragment of the IRF4/DUSP22 Break apart FISH Probe Kit labeled with CytoOrange covers some sequences downstream of the 3’ end of the IRF4 gene. The two probes are flanking sequences across the IRF4 and DUSP22 genes in which various breakpoints have been observed.