Archives: فروشگاه

Name: Flow Cytometry Antibody CD45-Pacific Blue™, Clone GA90

• Antibody CD45-PB^TM is a monoclonal antibody (mAb) labelled with Pacific Blue^TM designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD45 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Leukocytes are named according to structure as being either granulocytes or agranulocytes, and according to the function as either phagocytes or immunocyte. The granulocytes, which include neutrophils, basophils and eosinophils, are all phagocytes. Of the agranulocytes, the monocytes and macrophages are phagocytes whereas the lymphocytes are immunocytes. The granulocytes have granules in their cytoplasm, which contain enzymes which are capable of killing microorganisms and destroying debris ingested by the process of phagocytosis. The CYT-45PBZ mAb reacts with the leucocyte common antigen (LCA) complex, which is present on lymphocytes, monocytes, granulocytes, eosinophils, basophils and their progenitors. This reagent can be used in the characterization studies for immunophenotyping of leucocytes, which are widely applied in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemias, etc. Pacific BlueTM is excited with the violet laser (405nm) and emits at 550nm. This fluorochrome provides maximum resolution and narrow emission peaks, which results in little spectral overlap and minimal compensation requirements. In multicolor panel it is recommended the use of this fluorochrome combined with Orange Cytognos 515, which is also excited with the violet laser and emits at 515nm.

REAGENT COMPOSITION

Purified monoclonal CD45 antibody conjugated with Pacific Blue^TM, supplied in phosphate buffered saline with 0,09% sodium azide.

• Clone: GA90.
• Isotype: Mouse / IgG2a.
• Amount per 0,5 ml vial: 100 tests (5 µL mAb per determination)
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD45-PE-Cyanine5, Clone HI30

• Reagent CD45 is a monoclonal antibody (mAb) labelled with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD45 antigen by Flow Cytometry (FC). This reagent must be used by flow cytometry qualified personnel.

        SUMMARY AND EXPLANATION

Leukocytes are named according to structure as being either granulocytes or agranulocytes, and according to the function as either phagocytes or immunocyte. The granulocytes, which include neutrophils, basophils and eosinophils, are all phagocytes. Of the agranulocytes, the monocytes and macrophages are phagocytes whereas the lymphocytes are immunocytes. The granulocytes have granules in their cytoplasm, which contain enzymes which are capable of killing microorganisms and destroying debris ingested by the process of phagocytosis. The anti-CD45 mAb reacts with the leukocyte common antigen (LCA) complex, which is present on lymphocytes, monocytes, granulocytes, eosinophils, basophils and their progenitors. This reagent can be used in the characterization studies for immunophenotyping of leukocytes, which are widely applied in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemias, etc.

REAGENT COMPOSITION

Purified monoclonal CD45 antibody conjugated with different fluorochromes (see table above) and supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: HI30
• Isotype: Mouse / IgG1
• Purification: Affinity chromatography
• Amount per vial: 200/50 tests (5 or 3 µl mAb to 106 cells (see table above))
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD55-PE, Clone NaM16-4D3

• Antibody CD55-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD55 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Several key complement regulatory proteins regulate the activation of complement cascade preventing damage to autologous cells during an inflammatory reaction. Complement regulatory protein CD55 works down-regulating the complement cascade at the critical step of C3 and C5 activation. CD55 is expressed in all haematopoietic cells that circulate in contact with plasma complement proteins as well as on a wide spectrum of cells of non-haematopoietic origin and its overexpression was observed in several malignant tumour cells stopping them from being destroyed by complement activity. In paroxysmal nocturnal haemoglobinuria (PNH) GPI tail proteins domain have a synthetic defect which leads to a deficient expression of all GPI-anchored proteins (e.g. CD55 and CD59). For that reason, CD55 together with CD59 have been recurrently used in PNH diagnosis.

REAGENT COMPOSITION

The purified monoclonal CD55 Antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: NaM16-4D3
• Isotype: IgG1.
• Amount per 1 mL vial: 200 tests (5 µL mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD59-PE, Clone NaM172-2B5

• Antibody CD59-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD59 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Membrane-associated C-regulatory proteins are the major protection against Complement attack. CD59 (Protectin), a 19 kDa GPI-linked membrane glycoprotein is one of these regulatory proteins binding to the complement components C8 and C9 preventing the destruction of autologous cells. CD59 has a broadly distributed expression on all lymphocytes, monocytes, granulocytes, erythrocytes and in a variety of non-haematological cells. CD59 modulates T cell adhesion and plays a role in T cell activation (2, 3) . CD59 deficiency was already detected in clinical inflammatory disorders. Cells from patients with the haematological disorder paroxysmal nocturnal haemoglobinuria (PNH) are defective for GPI anchor synthesis and hence lack surface expression of CD59 and are highly susceptible to the lytic effects of Complement attack. For this reason CD59 together with CD55, another GPI-anchored protein, have been systematically used in diagnosis of PNH.

REAGENT COMPOSITION

The purified monoclonal CD59 Antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: NaM172-2B5
• Isotype: IgG1.
• Amount per 1 mL vial: 200 tests (5 µL mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD62L-FITC, Clone SK11

• Antibody CD62L-FITC is a monoclonal antibody (mAb) labelled with the tandem fluorescein isothiocyanate (FITC), designed for flow cytometry (FC) use as direct immunofluorescence reagent for the identification and enumeration of CD62L antigenexpressing cells. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

CD62L is a single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer’s patches. It also plays a role in leukocyte rolling on activated endothelial cells.

REAGENT COMPOSITION

Purified monoclonal CD62L Antibody conjugated with the tandem fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide and 0,2% (w/v) bovine serum albumin (BSA).

• Clone: SK-11
• Isotype: Mouse / IgG2a
• Purification: Affinity chromatography
• Amount per vial: 100 tests (5 µL mAb)
• Reagent is not considered sterile.

Name: Flow Cytometry Antibody CD81-APC-C750™, Clone M38

• Antibody Anti human CD81-APC-C750^TM is a monoclonal antibody (mAb) labelled with the tandem allophycocyanine-C750 (APCC750^TM) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD81 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

The CD81 (TAPA-1) antigen is expressed on normal lymphocytes, monocytes and eosinophils, but not on platelets and neutrophils. This antigen may be present in some cases as multimolecular complexes, in association with other members of the TM4 superfamily (CD37, CD53), or on the surface of B cells, in association with CD19 and/or CD21 and/or MHC class II antigens (3, 4). Most B lymphocytes, at all stages of cellular differentiation, express CD81 at relatively high levels. Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

Purified monoclonal CD81 antibody conjugated with the tandem allophycocyanine-C750 (APC-C750TM), supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: M38.
• Isotype: Mouse / IgG1.
• Amount per 0,15 ml vial: 50 tests (3 µL mAb per determination)
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD81-APC-C750™, Clone M38

• Antibody Anti human CD81-APC-C750^TM is a monoclonal antibody (mAb) labelled with the tandem allophycocyanine-C750 (APCC750^TM) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD81 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

The CD81 (TAPA-1) antigen is expressed on normal lymphocytes, monocytes and eosinophils, but not on platelets and neutrophils. This antigen may be present in some cases as multimolecular complexes, in association with other members of the TM4 superfamily (CD37, CD53), or on the surface of B cells, in association with CD19 and/or CD21 and/or MHC class II antigens (3, 4). Most B lymphocytes, at all stages of cellular differentiation, express CD81 at relatively high levels. Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

Purified monoclonal CD81 antibody conjugated with the tandem allophycocyanine-C750 (APC-C750TM), supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: M38.
• Isotype: Mouse / IgG1.
• Amount per 0,15 ml vial: 50 tests (3 µL mAb per determination)
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD81-FITC, Clone M38

• Antibody Anti human CD81-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD81 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

The CD81 (TAPA-1) antigen is expressed on normal lymphocytes, monocytes and eosinophils, but not on platelets and neutrophils. This antigen may be present in some cases as multimolecular complexes, in association with other members of the TM4 superfamily (CD37, CD53), or on the surface of B cells, in association with CD19 and/or CD21 and/or MHC class II antigens. Most B lymphocytes, at all stages of cellular differentiation, express CD81 at relatively high levels. Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

Purified monoclonal CD81 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: M38.
• Isotype: Mouse / IgG1.
• Amount per vial: 100 tests (5 µL mAb per determination)
• Reagents are not considered sterile.