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Name: Flow Cytometry Antibody CD31-FITC, Clone WM59

• Antibody CD31-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD31 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. The CD31 antigen is expressed on endothelial cells, platelets, T-lymphocyte subsets, monocytes, and granulocytes. This antigen is the only known member of the CAM family to be expressed on platelets. The antigen is localized at regions of cell–cell contacts and may function as an adhesion molecule, mediating adhesion between leucocytes/endothelial cells, leucocytes/platelets, and endothelial cells/ endothelial cells.

REAGENT COMPOSITION

The purified monoclonal CD31 Antibody conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: WM59.
• Isotype: IgG1.
• Amount per 1 ml vial: 200 tests (5 µl MAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD35-FITC, Clone E11

• Antibody CD35-FITC is a monoclonal antibody (mAb) labelled with Fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD35 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. CD35, also known as complement receptor 1 (CR1), is a N-glycosylated member of the RCA (regulators of complement activation) family of proteins. It is expressed by erythrocytes, granulocytes, monocytes, dendritic cells, B cells and some NK cells. CD35 is also expressed leukemic cells in a subset of AML (acute myeloid leukemia) patients. CD35 has been selected by EuroFlow consortium in its AML panel .

REAGENT COMPOSITION

The purified monoclonal CD35 Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: E11
• Isotype: IgG1.
• Amount per vial: 100 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD36-FITC, Clone CLB-IVC7

• Antibody CD36-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD36 antigen by flow cytometry (FC). This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

The CYT-36F3 mAb reacts with an 88kDa single chain glycoprotein expressed by platelets and monocytes/macrophages. The molecule is the receptor for thrombospondin and also acts as a receptor on endothelium for malaria parasitized RBCs. CD36 is expressed early during erythroid maturation, but is not specific to erythroid cells, since it is also positive in precursor cells of the monocytic and megakaryocytic lineages. Analysis of the expression of this antigen is useful for the characterization of myelodysplastic syndromes and some AML.

REAGENT COMPOSITION

Purified monoclonal CD36 Antibody conjugated with fluorescein isothiocyanate (FITC), is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: CLB-IVC7
• Isotype: IgG1
• Purification: Affinity chromatography
• Amount per vial: 100 tests (5 µL mAb to 106 cells)
• Reagents are not considered sterile

Name: Flow Cytometry Antibody CD38-APC-C750™, Clone LD38

• Antibody CD38 is a monoclonal antibody (mAb) designed for flow cytometry (FC) use as direct (or indirect for the CD38-PURE) immunofluorescence reagent for the identification and enumeration of CD38 antigen-expressing cells. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

CD38 antigen is a type II transmembrane glycoprotein expressed at variable levels on the majority of hematopoietic cells (during early differentiation and activation) and in some non-hematopoietic tissues. It is expressed at high levels on plasma cells (CD38+++) and, due to its strong reactivity, CD38 is considered a “specific plasma cell marker”; but it is also present on thymocytes and activated T cells. CD38 has been reported to play a role as regulator (positive and negative) of cell activation and proliferation, and to be involved in adhesion between lymphocytes and endothelial cells. CD38 antibody is used in FC for diagnosis and monitoring of plasma cell disorders (e.g. multiple myeloma, MGUS, plasma cell leukemia, etc.) .The simultaneous assessment of the expression of CD38 and CD138 represents the best combination of markers for the identification of plasma cells. Plasma cells (CD38+++) are expressed as a percentage of leucocytes present in a sample. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

CD38 antibody is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0,09% (m/v) sodium azide.

Clone: LD38.

Isotype: Mouse / IgG1.

Amount per vial: 50, 100 or 200 tests (3,5 or 10 µl mAb per determination (see table above)).

Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD38-Pure, Clone EBVCS-5

• Antibody CD38 is a monoclonal antibody (mAb) designed for flow cytometry (FC) use as direct (or indirect for the CD38-PURE) immunofluorescence reagent for the identification and enumeration of CD38 antigen-expressing cells. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

CD38 antigen is a type II transmembrane glycoprotein expressed at variable levels on the majority of hematopoietic cells (during early differentiation and activation) and in some non-hematopoietic tissues. It is expressed at high levels on plasma cells (CD38+++) and, due to its strong reactivity, CD38 is considered a “specific plasma cell marker”; but it is also present on thymocytes and activated T cells. CD38 has been reported to play a role as regulator (positive and negative) of cell activation and proliferation, and to be involved in adhesion between lymphocytes and endothelial cells. CD38 antibody is used in FC for diagnosis and monitoring of plasma cell disorders (e.g. multiple myeloma, MGUS, plasma cell leukemia, etc.). The simultaneous assessment of the expression of CD38 and CD138 represents the best combination of markers for the identification of plasma cells. Plasma cells (CD38+++) are expressed as a percentage of leucocytes present in a sample. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

CD38 antibody is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0,09% (m/v) sodium azide.

• Clone: LD38.
• Isotype: Mouse / IgG1.
• Amount per vial: 50, 100 or 200 tests (3, 5 or 10 µl mAb per determination (see table above)).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD39-PE, Clone TU66

• Antibody CD39-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD39 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression.

CD39 is a cell surface protein member of the ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) family. It is a 78 kDa cell surface enzyme also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1). CD39 is expressed by regulatory T cells, mantle zone B cells, activated T cells, NK cells, macrophages, dendritic cells, neurons, endothelial cells and platelets. It is thought that CD39 allows Treg cells to enter inflamed areas where high levels of ATP are present. In the B-CLPD (B-cell chronic lymphoproliferative diseases) EuroFlow panel, CD39 is used to improve differential diagnosis between subgroups of lymphoplasmacytic lymphoma, marginal zone lymphoma, follicular lymphoma and diffuse large B-cell lymphoma cases.

REAGENT COMPOSITION

The purified monoclonal CD39 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: TU66.
• Isotype: IgG2b.
• Amount per vial: 100 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD42-FITC, Clone GRP-P

• Antibody CD42-FITC is a monoclonal antibody (mAb) labelled with Fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD42 antigen-expressing cells. This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

CD42a, also known as glycoprotein 9 (GPIX), is a 23kDa surface marker expressed by platelets and megakaryocytes. CD42a is critical in thrombus formation. Alterations in CD42a gene cause giant platelet disease or Bernard-Soulier syndrome. CD42a has been selected by EuroFlow consortium in its AML (acute myeloid leukemia) panel.

REAGENT COMPOSITION

The purified monoclonal CD42a Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphatebuffered saline (PBS) containing 0.09% sodium azide.

• Clone: GRP-P
• Isotype: IgG1.
• Amount per vial: 100 tests (5 mL mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD43-FITC, Clone DFT1

• Antibody CD43-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothicyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD43 antigen by Flow Cytometry (FC).

SUMMARY AND EXPLANATION

FC is a powerful tool for the analytical and quantitative characterization of cells which provides rapid, quantitative and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cells in liquid suspension that have been incubated with fluorescently-labeled antibodies directed against specific cellular proteins. The relative fluorescence intensity of the positive cells indicates the amount of antibody bound to specific binding sites on the cells, and therefore provides a relative measure of antigen expression. CYT-43F2 mAb recognizes the CD43 antigen, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. CD43 exerts dual function in cell adhesion and anti-adhesion, lectin binding, as well as co-stimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors .During T-cell activation is actively removed from the T-Cell APC (antigen presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. The expression of CD43 is found on most leukocytes, except resting B lymphocytes.

REAGENT COMPOSITION

Purified monoclonal CD43 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with ≤0.09% (m/v) sodium azide.

• Clone: DFT1
• Isotype: IgG1
• Amount per vial: 200 tests (5µl mAb per determination)
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD45-APC-C750™ (RUO), Clone HI30

• Antibody Reagent CD45 is a monoclonal antibody (mAb) labelled with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD45 antigen by Flow Cytometry (FC). This reagent must be used by flow cytometry qualified personnel.

        SUMMARY AND EXPLANATION

Leukocytes are named according to structure as being either granulocytes or agranulocytes, and according to the function as either phagocytes or immunocyte. The granulocytes, which include neutrophils, basophils and eosinophils, are all phagocytes. Of the agranulocytes, the monocytes and macrophages are phagocytes whereas the lymphocytes are immunocytes. The granulocytes have granules in their cytoplasm, which contain enzymes which are capable of killing microorganisms and destroying debris ingested by the process of phagocytosis. The anti-CD45 mAb reacts with the leukocyte common antigen (LCA) complex, which is present on lymphocytes, monocytes, granulocytes, eosinophils, basophils and their progenitors. This reagent can be used in the characterization studies for immunophenotyping of leukocytes, which are widely applied in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemias, etc.

REAGENT COMPOSITION

Purified monoclonal CD45 antibody conjugated with different fluorochromes (see table above) and supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide. Clone: HI30

• Isotype: Mouse / IgG1
• Purification: Affinity chromatography
• Amount per vial: 200/50 tests (5 or 3 mL mAb to 106 cells (see table above))
• Reagents are not considered sterile.