دسته بندی: آنتی بادیهای ایمونوهیستوشیمی

Name: IgG Antibody Clone E20-V

Description and applications: The antibody labels the IgGs contained in the plasma cells and their precursors. In general, membrane bound immunoglobulins, extracellular immunoglobulins bound to connective tissue or blood vessels, and immunocomplexes can only be demonstrated in frozen tissues. Plasma cells may not
be intensely stained in frozen tissue since the immunoglobulins are distributed diffusely through the cytoplasm of these cells. This antibody is useful for identifying leukemias, plasmacytomas and B cells derived from Hodgkin lymphomas. Due to the restrictions on the expression of light and heavy chains in these diseases, demonstration of B-cell lymphomas is possible with clonal rearangements studies. Another application is

Composition: Anti-human IgG rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: IMP 3 Antibody Clone EP286

Description and applications: IMP-3, known as Insulin-like growth factor 2 (IGF-II) mRNA-binding protein 3, is an oncofetal protein that stabilizes IGF-II mRNA for trafficking and plays an important role in cell growth and migration. This 65- 70 kDa protein is expressed normally in developing tissues during early embryogenesis in a variety of fetal tissues including the liver, lung kidney, thymus, and placenta, but at low or undetectable levels in normal adult tissues. Recent studies have demonstrated IMP-3 expression in various malignant tumors of the lung, gastrointestinal tract, liver, endometrium, and bladder, while undetectable in adjacent benign tissues. IMP-3 may have a critical role in tumor proliferation, invasion and metastasis, and has been suggested to be an independent marker for poor prognosis in patients with clear cell carcinomas.

Composition: Anti-human IMP 3 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Inhibin Alpha Antibody (Clone R1)

Description and applications: This antibody recognizes the 32kDa alpha subunit of human inhibin. Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumors, placental and gestational trophoblastic lesions and sex cord stromal tumors.

Composition: anti-human Inhibin alpha mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Insulin Antibody Clone EP125

Description and applications: Insulin is a hormone that regulates glucose homeostasis. It increases cell permeability to monosaccharides, amino acids and fatty acids, and it accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. It is synthesized in the beta cell of the pancreas. The antibody labels both normal and neoplastic insulinproducing cells. It is useful in identifying insulinoma.

Composition: Anti-human Insulin rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Keratin 18 Antibody Clone DC-10

Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins , for example keratin brand AE1 keratins 10, 14 , 15, 16 and 19. This antibody reacts with the acid intermediate filament protein of 45 kDa keratin, identified as Keratin 18. Keratin 18 is generally co-expressed with the keratin 8 in most simple gland epithelia. In general, the keratin 18 does not react with squamous multilayered epithelium. In neoplastic tissues, this antibody reacts with different benign and malignant epithelial lesions. Most adenocarcinomas and basal cell carcinomas express this keratin, whereas it is absent in squamous cell carcinomas.

Composition: anti-Keratin 18 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The amount of the active antibody in the sample is 3,3 μg/mL..

Immunogen: Human breast cancer PMC 42 cells.

Name: LIN-28 Antibody Clone EP150

Description and applications: LIN28 is a highly conserved, RNA-binding protein (RBP). It plays an important role as a translational enhancer, leading specific mRNAs to polysomes and therefore increasing the competence of protein synthesis. LIN28 was identified as a negative regulator of miRNA biogenesis and suggested to play a central role in blocking miRNAmediated differentiation in stem cells and certain cancers. LIN28 is expressed by various undifferentiated embryonic cell types. Anti- LIN28 has been used as a sensitive marker for germ cell tumors. The positive staining of LIN28 in yolk sac tumors showed an advantage over OCT4, which is negative in these tumors.

Composition: Anti-human LIN-28 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Mouse anti-human MAP-2 Monoclonal Antibody clone AP20

Description and applications: MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP- 2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds.

Composition: Anti-human MAP-2 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide 

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Name: Rabbit anti-human Myo-D1 Monoclonal Antibody clone EP212

Description and applications: MyoD1 is a protein with a key role in regulating muscle differentiation. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. MyoD1 is expressed in developing skeletal muscle tissue but faintly in adult skeletal muscle. In abnormal tissues, it labels tumour cell in rhabdomyosarcoma. MyoD1 is one of the earliest markers of myogenic commitment. Antibody to MyoD1 has been useful to differentiate rhabdomyosarcomas from other tumors. It is a sensitive and specific marker for myogenic differentiation

Composition: anti-human Myo-D1 rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Name: Mast Cell Tryptase Antibody Clone EP259

Description and applications: Human mast cell tryptase, comprises a family of trypsin-like neutral serine proteinases that are predominantly expressed in mast cells. Tryptase has effects on peptides, proteins, cells, and tissues, and many of these actions can ultimately contribute to asthma symptoms. Mast-cell tryptase is found in mast-cell granules and has also been reported to be expressed by peripheral blood basophils at low level. Tryptase has been used as a marker of mast cell activation.

Composition: Anti-human Mast Cell Tryptase rabbit monoclonal antibody purified from serum and prepared in 10mM
PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.