فلوسایتومتری

Name: Flow Cytometry Antibody Cytokeratin18-FITC, Clone Ks18.04

• Antibody Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human cytokeratin 18) of all simple type epithelium and basal cells of many squamous, nonepidermal epithelium.

Suitable for:

– Immunohistochemistry of frozen and paraffinembedded tissue and cytological material (Work dilution 1:10)

– Cell labeling for later analysis by flow cytometry – This reagent must be used by flow cytometry qualified personal.

REAGENT COMPOSITION

Purified mouse monoclonal antibody to cytokeratin 18-FITC conjugate, supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and ≤0.09% (m/v) sodium azide.

• Clone: Ks18.04
• Isotype: IgG1
• Purification: Protein A affinity chromatography
• Amount per vial: 0.25 ml

Name: Flow Cytometry Antibody FMC7-FITC, Clone FMC7

• Antibody FMC7-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of FMC7 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. The FMC-7 antibody detects a glycoprotein of 105kD found on circulating B-lymphocytes. Studies on normal lymphocytes, leukaemic cells and cell lines indicate that FMC-7 is a marker for a limited segment of the B cell maturation pathway. This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukaemia (B-CLL), prolymphocytic leukaemia (PLL) and other B cell neoplasias. It stains peripheral blood B lymphocytes and tonsil B lymphocytes. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells. The FMC-7 antibody reacts with HRIK and Raji cell lines.

REAGENT COMPOSITION

The purified monoclonal FMC7 antibody conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: FMC7
• Isotype: IgM
• Amount per 1 ml vial: 200 tests (5 µl mAb per determination)
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody HLA-B7-PE, Clone BB7.1

• HLA-B7-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of HLA-B7 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. CYT-HLAIB7PE binds to the HLA-B7 antigen and does not cross react with HLAB27 or other related HLA antigens. HLAB27 and HLA-B7 antigens are expressed in 7% and 22% of individuals of Caucasian origin. This mixture of antibodies permits the characterization of HLAB27 specificity in the HLA class I allotype in patients suffering from inflammatory disorders affecting the sacroiliac and intervertebral joints. HLAB27-PE antibody can be used to distinguish true HLA-B27 positives (HLA-B27+ /HLA-B7- ) from false HLA-B27 positives (HLAB7dim+/HLA-B7+).

REAGENT COMPOSITION

The purified monoclonal HLA-B7 Antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: BB7.1
• Isotype: IgG1.
• Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody HLA-DR-Pacific Blue^TM, Clone L243

• HLA-DR is a monoclonal antibody (mAb) labelled with Pacific Blue^TM designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of HLA-DR antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. The main function of human leucocyte antigen (HLA) molecules is to present antigenic peptides to the T-cell receptor, thereby regulating the induction of the immune response. The HLA molecules are encoded by a cluster of tightly linked genes located on the short arm of chromosome 6. Three classes of HLA molecules (I, II and III) have been denoted. Human class II genes are located in the HLA-D region, consisting of three families called DQ, DP and DR. The products of class II genes form a heterodimeric transmembrane protein, consisting of a heavy α-chain and a light βchain. The DR α-chain is expressed from one non-polymorphic gene, whereas the DR β- chain originates from nine highly polymorphic genes. HLA-DR antigen is constitutively expressed on antigen-presenting cells, such as B lymphocytes, monocytes and dendritic cells but can also be detected on activated T lymphocytes and activated granulocytes. The antigen has been found expressed in cases of different types of acute lymphoblastic leukaemias, acute myeloid leukaemias, chronic lymphoblastic leukaemias, chronic myeloid leukaemias and B- and T-cell non-Hodgkin’s leukaemias. However, the antigen is normally not present on non-haematopoietic tumours and multiple myelomas.

REAGENT COMPOSITION

The purified monoclonal HLA-DR Antibody conjugated with Pacific BlueTM is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: L243
• Isotype: IgG2a.
• Amount per vial: 100 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody HLA-DR-PE, Clone L243

• HLA-DR-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of HLA-DR antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

The main function of human leucocyte antigen (HLA) molecules is to present antigenic peptides to the T-cell receptor, thereby regulating the induction of the immune response. The HLA molecules are encoded by a cluster of tightly linked genes located on the short arm of chromosome 6. Three classes of HLA molecules (I, II and III) have been denoted. Human class II genes are located in the HLA-D region, consisting of three families called DQ, DP and DR). The products of class II genes form a heterodimeric transmembrane protein, consisting of a heavy α-chain and a light βchain. The DR α-chain is expressed from one non-polymorphic gene, whereas the DR β- chain originates from nine highly polymorphic genes. HLA-DR antigen is constitutively expressed on antigen-presenting cells, such as B lymphocytes, monocytes and dendritic cells but can also be detected on activated T lymphocytes and activated granulocytes. The antigen has been found expressed in cases of different types of acute lymphoblastic leukaemias, acute myeloid leukaemias, chronic lymphoblastic leukaemias, chronic myeloid leukaemias and B- and T-cell non-Hodgkin’s leukaemias. However, the antigen is normally not present on non-haematopoietic tumours and multiple myelomas.

REAGENT COMPOSITION

The purified monoclonal HLA-DR Antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: L243
• Isotype: IgG2a.
• Amount per 1 mL vial: 200 tests (5 µL mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody Kappa-APC, Clone Polyclonal

• Antibody Kappa-APC is a polyclonal antibody (pAb) labelled with allophycocyanine (APC) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express Kappa immunoglobulin Light Chains by flow cytometry. This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and Natural Killer cells (NK). B lymphocytes are the producers of antibodies and mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. Most B cells, with the exception of pre-B progenitors, pre B- cells, and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type (Kappa or Lambda). In normal peripheral blood and lymph nodes, there is a mixture of Kappa positive and Lambda positive cells, with two-thirds of the cells expressing Kappa and one-third expressing Lambda. Since lymphoid neoplasms are usually clonal expansions of a single cell, malignant cells uniformly express the same Light Chain isotype. Neoplastic B cell lymphoprolipherative disorders can frequently be suspected based on the demonstration of a marker predominance of cells expressing a single Light Chain type. The Kappa immunoglobulin Light Chain count is generally expressed as a percentage of B cells present in the sample that can be determined by flow cytometry based on its positive expression of CD19.

REAGENT COMPOSITION

Purified polyclonal antibody Anti-human Kappa Light Chains, Goat F(ab’)2, conjugated with allophycocyanine (APC), supplied in phosphate buffered saline with 0,09% sodium azide and 1% (w/v) bovine serum albumin (BSA).

• Clone: Polyclonal
• Isotype: Goat / Polyclonal
• Amount per vial: 100 tests (5 µL/Test).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody Kappa-PerCP-Cyanine5.5, Clone Polyclonal

• Antibody Kappa-PerCP-Cyanine5.5 is a polyclonal antibody (pAb) labelled with tandem Peridin chlorophyll protein-Cyanine 5.5 (PerCP-Cyanine5.5) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells that express human Kappa immunoglobulin Light Chains by Flow Cytometry (FC). Antibodies to Kappa Light Chains are useful for the identification of clonal excess in B-cell lymphoproliferative disorders together with a panel of other antibodies. Anti-Kappa Light Chains reacts with human free Kappa chains as well as Kappa chains in intact immunoglobulin molecules. This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and Natural Killer cells (NK). B lymphocytes are the producers of antibodies and mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. Most B cells, with the exception of pre-B progenitors, pre-B cells, and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type (Kappa or Lambda). In normal peripheral blood and lymph nodes, there is a mixture of Kappa positive and Lambda positive cells, with two-thirds of the cells expressing Kappa and one-third expressing Lambda. The Kappa immunoglobulin Light Chain count is generally expressed as a percentage of B cells present in the sample which can be determined by flow cytometry based on its positive expression of CD19. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure and the combination of markers used to identify the target cell population.

REAGENT COMPOSITION

Purified polyclonal antibody anti-human Kappa Light Chains, Goat F(ab’)2, conjugated with tandem Peridin chlorophyll protein-Cyanine 5.5 (PerCP-Cyanine5.5), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.

• Amount per vial: 50 tests (3µL pAb to 106 cells).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody Lambda-APC-C750^TM, Clone Polyclonal

• Antibody Lambda-APC-C750^TM is a polyclonal antibody (pAb) labelled with R-Phycoerythrin (PE) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells that express human Lambda immunoglobulin Light Chains by Flow Cytometry (FC). Antibodies to Lambda Light Chains are useful for the identification of clonal excess in B-cell lymphoproliferative disorders together with a panel of other antibodies. Anti-Lambda Light Chains reacts with free Lambda chains as well as Lambda chains in intact immunoglobulin molecules. This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and Natural Killer cells (NK). B lymphocytes are the producers of antibodies and mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. Most B cells, with the exception of pre-B progenitors, pre B-cells, and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type (Kappa or Lambda). In normal peripheral blood and lymph nodes, there is a mixture of Kappa positive and Lambda positive cells, with two-thirds of the cells expressing Kappa and one-third expressing Lambda. Since lymphoid neoplasms are usually clonal expansions of a single cell, malignant cells uniformly express the same Light Chain isotype. Neoplastic B cell lymphoprolipherative disorders can frequently be suspected based on the demonstration of a marker predominance of cells expressing a single Light Chain type. The Lambda immunoglobulin Light Chain count is generally expressed as a percentage of B cells present in the sample that can be determined by FC based on its positive expression of CD19. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure and the combination of markers used to identify the target cell population.

REAGENT COMPOSITION

Purified polyclonal antibody Anti-human Lambda Light Chains, Goat F(ab’)2, conjugated with R-Phycoerythrin (PE), supplied in phosphate buffered saline with 0.09% (m/v) sodium azide.

• Amount per 0,5 ml vial: 100 tests (5 µl pAb to 106 cells).
• Reagents are not considered sterile.