Name: Flow Cytometry Antibody CD2-FITC, Clone RPA-2.10

• Antibody CD2-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD2 antigen by flow cytometry (FC). CD2 can be considered a pan-T cell antigen, as it is expressed on the surface of all normal Tlymphocytes. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

 The CYT-2F7 mAb recognizes the 50kDa lymphocyte surface antigen expressed on all peripheral blood T lymphocytes, the majority of thymocytes and malignant cells of T cell origin, including T ALL cells. CD2 is a useful marker in the assessment of lymphoid malignancies as it is expressed in the majority of precursor and posthymic lymphomas and leukemias. In some neoplastic T-cell populations e.g. in peripheral T cell lymphomas, CD2 may be aberrantly deleted.

 REAGENT COMPOSITION

Purified monoclonal CD2 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide.

• Clone: RPA-2.10
• Isotype: IgG1
• Purification: Affinity chromatography
• Amount per 1 mL vial: 200 tests (5 µL mAb to 106 cells)
• Reagents are not considered sterile.

+Name: CD3-FITC, Clone (UCHT-1)

• Antibody CD3 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personnel.
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SUMMARY AND EXPLANATION

The anti-CD recognizes the CD3 antigen present in T cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of posttransplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. The T lymphocyte (CD3+) count is generally expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by flow cytometry based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

Purified monoclonal CD3 antibody conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: UCHT-1
• Isotypes: Mouse / IgG1
• Purification: Affinity
• chromatography Amount per vial: 50 or 200 tests (3 or 5 µl mAb to 106 cells).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD3-PE, Clone UCHT-1

• Antibody CD3 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

The anti-CD recognizes the CD3 antigen present in T cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of posttransplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. The T lymphocyte (CD3+) count is generally expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by flow cytometry based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

Purified monoclonal CD3 antibody conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: UCHT-1
• Isotypes: Mouse / IgG1
• Purification: Affinity chromatography
• Amount per vial: 50 or 200 tests (3 or 5 µl mAb to 106 cells) (see table above).
• Reagents are not considered sterile.

Name: Flow Cytometry AntibodyCD3-APC-C750™, Clone UCHT-1

• Antibody CD3 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

The anti-CD recognizes the CD3 antigen present in T cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of posttransplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. The T lymphocyte (CD3+) count is generally expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by flow cytometry based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

 REAGENT COMPOSITION

Purified monoclonal CD3 antibody conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: UCHT-1
• Isotypes: Mouse / IgG1
• Purification: Affinity chromatography
• Amount per vial: 50 or 200 tests (3 or 5 µl mAb to 106 cells).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD3-Pacific Blue, Clone UCHT-1

• Antibody CD3-Pacific Blue^TM is a monoclonal antibody (mAb) labelled with Pacific Blue^TM designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

 Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. CYT-3PBZ mAb recognizes the CD3 antigen present in T cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. Pacific Blue^TM, is excited with the violet laser (405nm) and emits at 550nm. This fluorochrome provides maximum resolution and narrow emission peaks, which results in little spectral overlap and minimal compensation requirements. In multicolor panel it is recommended the use of this fluorochrome combined with Orange Cytognos 515, which is also excited with the violet laser and emits at 515nm.

REAGENT COMPOSITION

 Purified monoclonal CD3 antibody antibody conjugated with Pacific BlueTM, supplied in phosphate buffered saline with 0, 09% sodium azide.

• Clone: UCHT1.
• Isotype: Mouse / IgG1.
• Amount per 0,5 mL vial: 100 tests (5 µL mAb per determination).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD5-FITC, Clone MCD5

• Antibody CD5-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD5 antigen by flow cytometry (FC). CD5 is a useful marker for the identification of certain lymphoprolipherative syndromes when is used within an extended immunophenotyping panel. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

The CYT-5F4 mAb recognizes a 67 kDa antigen expressed by a majority of thymocytes and mature T cells and a subset of B cells. CD5 is expressed at low density on thymocytes and at high density on all mature T lymphocytes. The majority of T cell malignancies expresses CD5, and almost 85% of T cell acute lymphoblastic leukemias are CD5 positive. Further, CD5 is expressed at low density on a small subset of mature B lymphocytes, which is expanded in several autoimmune disorders as well as in some B-cell derived lymphoproliferative disorders such as chronic lymphocytic leukemia (CLL) and centrocytic leukemia.

REAGENT COMPOSITION

Purified monoclonal CD5 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide.

• Clone: MCD5
• Isotype: IgG2b
• Purification: Affinity chromatography
• Amount per 1 ml vial: 200 tests (5 ml mAb to 106 cells)
• Reagents are not considered sterile

Name: Flow Cytometry Antibody CD5-PE, Clone MCD5

• Antibody CD5-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD5 antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). CYT-5PE4 mAb recognizes a 67 kDa antigen expressed by a majority of thymocytes and mature T cells and a subset of B cells CD5 is expressed at low density on thymocytes and at high density on all mature T lymphocytes. The majority of T cell malignancies express CD5, and almost 85% of T cell acute lymphoblastic leukemias are CD5 positive. Further, CD5 is expressed at low density on a small subset of mature B lymphocytes, which is expanded in several autoimmune disorders as well as in some B-cell derived lymphoproliferative disorders such as chronic lymphocytic leukemia (CLL) and centrocytic leukemia  .

REAGENT COMPOSITION

The purified monoclonal CD5 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.09% sodium azid.

• Clone: MCD5.
• Isotype: IgG2b.
• Amount per 1 mL vial: 200 tests (5 µL mAb per determination).
• Reagent is considered non-sterile.