Category: DAB
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DABImmunoperoxidase DAB Kit (Dark Brown)
Available Size : 100 test / 500 test
Kit components:
DAB Substrate Buffer (READY-TO-USE)
DAB Chromogen Concentrate (READY-TO-USE)
DAB enhancer (READY-TO-USE)Spcificity, Interferences and Limitations:
The Immunoperoxidase DAB Kit (Dark Brown) is preferred for immunohistochemical staining methods, based
on peroxidase marked antibodies and in situ hybridization.
Once the DAB oxidation has occurred, a brown insoluble precipitate is obtained. An increase or decrease in the
ratio between the chromogen and its substrate volumes, as well as exposure to bright light, can affect the
degree of specific staining and background of the final reaction. For this reason it is recommended that the
staining should be made in low light conditions. -
DABMaster Polymer Plus Detection System (Peroxidase)
Available Size : 100 test / 500 test / 1250 test
Intended use: Product for in vitro diagnostic use.
Master Polymer Plus Detection System is developed based on the micro-polymer technology and is the result of a decade of experience in polymer systems for immunohistochemistry. This detection system has demonstrated higher sensitivity with an increase in the antigen-antibody binding signal. The novel technology of micro-polymers provides greater advantages than conventional immunohistochemical techniques and facilitates the detection of antigens in different cellular compartments (nucleus, cytoplasm and cell membrane) due to the smaller size of the molecule. It also eliminates background staining caused by nonspecific binding of endogenous biotin molecules as the Master Polymer Plus Detection System staining procedure is not based on the avidin / biotin reaction.
– Primary monoclonal and polyclonal antibodies obtained in mouse
– Primary monoclonal and polyclonal antibodies obtained in rabbit. -
DABکیت 2Steps HRP Detection System برند PathoSage
Intended use:
PathoSage 2 Steps HRP Detection System Peroxidase Goat Anti-Mouse/Rabbit IgG HRP with DAB, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
Specification:
PathoSage 2 Steps HRP Detection System is good for low antigen expression levels and primary antibody dilution maximization. They consist of an amplifier antibody followed by an affinity-purified and cross-adsorbed polymer reagent to ensure high sensitivity and low background.
The PathoSage 5 Component Detection Kit includes Peroxidase solution. Peroxidazed blocking solution is a highly stable form of hydrogen peroxide for blocking endogenous peroxidase. It is very effective for blocking non-specific staining in red blood cells. It is non-flammable, safer and less toxic when compared to hydrogen peroxide/methanol formulations.
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DABکیت 1Step HRP Detection System برند PathoSage
Intended use:
PathoSage 1 step HRP Detection system Peroxidase Goat Anti-Mouse/Rabbit IgG with DAB, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
Specification:
PathoSage 1 step HRP Detection systems are good for single antigen staining, sequential double staining, and cross-reactivity reduction. These systems consist of micropolymers attached to highly cross-adsorbed, affinity-purified secondary antibodies that penetrate more deeply into thicker sections. These systems therefore provide specific primary antibody binding and avoid steric hindrance concerns. One-step systems also have a shorter assay time, so you get crisp images faster.
PathoSage 1 step detection system, peroxidase Goat Anti-Mouse/Rabbit HRP plus DAB, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU.