CISH Ancillaries
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اقلام مصرفی سیشچسب فیکسوگام Rubber Cement Fixogum
Read moreRubber Cement Fixogum is an elastic adhesive, ideal for “in situ” hybridization techniques for sealing the
coverslip on the slide once the probe is applied. -
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اقلام مصرفی سیشTBS Tween 20 Buffer 10X
Read moreProduct Composition: The composition of the 10x concentrated product is: Tris-HCl 500 mM, NaCl 3M, 0.5 % Tween 20, pH 7.5.
Recommendations for Use:
Before start using the product: This solution is 10x concentrated, so before its use it must be diluted
1:10 with double-distilled or deionized water (1 part of buffer and 9 parts of water). -
اقلام مصرفی سیشEDTA Buffer 10X pH8
Read moreProduct Composition: 10mM EDTA pH8.0.
Intended Use: Product for in vitro diagnostic use
Recommendations for Use:
Before use: The EDTA solution is 10x concentrated and therefore before use it should be diluted in a 1:10 range with distilled or deionized water (one part EDTA buffer and 9 parts water).
-Cut and mount sections on charged slides or silane coated.-Dewaxing (using xylene or substitute) and rehydrate the tissue sections thru decreasing series of alcohol. Then follow with the step 3 and 4.
-Unmasking: various alternative methods could be used to carry out the antigen retrieval
process using the diluted EDTA buffer-Remove the sections and place them into the usual washing buffer (recommended TBS / TBS-Tween pH 7.5 – reference MAD-004077R)
-Proceed with the immunostaining protocol as usual.