Lymphoma Kits

Kit characteristics
Contains an amplification mix for each one of the segments IgK (Vκ-Jκ, Vκ-Kde and intronRSS-Kde
rearrangements), IgL (Vλ-Jλ), and an internal control for verifying DNA quality (CI).
The amplification mixtures are in “monotest” form in colour-identified PCR tubes of 0.2 ml.
All the mixtures include primers labelled at the 5’ end with 6-FAM fluorochrome, which allows automatic
analysis of fragments by capillary electrophoresis (ABI PRISM® 310 Genetic Analyzer, AB 3130, 3130xl,
3500 y 3500xL Genetic Analyzers).
All amplifications can be carried out in the thermocycler using a single programme.
The enzyme Phire® Hot Start II DNA Polymerase is supplied in the kit.
Positive controls for clonal and polyclonal DNA are included.

REAGENT                                               COMPONENTS                                                          QUANTITY
Amplification mixtures                            IgK-A (6-FAM)                                                          20 PCR tubes blue (46 μl)
(monotest in tubes of 0.2)                      (mix of oligonucleotides from regions Vκ
and Jκ of IgK gene, dNTP and buffer solution)

                                                           IgK-B (6-FAM)                                                           20 PCR tubes:orange (46 μl)
(mix of oligonucleotides from regions Vκ,
intron-RSS, and Kde of IgK gene, dNTP and buffer solution)

IgL (6-FAM)                                                               20 PCR tubes green (46 μl)
(mix of oligonucleotides from regions Vλ-
Jλ of IgL gene, dNTP and buffer solution)

Internal control mix (CI) (6-FAM)                                 20 PCR tubes yellow (46 μl)
(oligonucleotides specific for exon 5 of p53
gene, dNTP and buffer solution)

DNA polymerase                                       Phire® Hot Start II                                                 2 x 60 μl
DNA Polymerase*

Clonal IgK-IgL positive control DNA                           1 x 50 μl
(50 μg/ml)

Positive control DNAs                                Polyclonal positive control DNA                                1 x 50 μl
(100 μg/ml)

Kit characteristics

Contains two reaction mixtures (Mixes A and B) for amplification of the Vß-Jß region and a mixture (Mix C) for
Dß-Jß region of the TCRß gene with an internal amplification control for checking the quality of the DNA.

The amplification mixtures are in “monotest” form in colour-identified PCR tubes of 0.2 – 0.5 ml.

All the PCR mixtures include primers marked at their 5’ end with 6-FAM fluorochrome, allowing automatic
analysis of fragments by capillary electrophoresis using GeneScan.

All amplifications can be carried out in the thermocycler using a single programme.

The enzyme Phire® Hot Start II DNA Polymerase is supplied in the kit.

Positive controls for clonal and polyclonal DNA are included.

Kit characteristics

Contains two multiplex reaction mixtures (Mixes A and B) for the V-J region of the TCRgamma gene with an
internal amplification control for checking the quality of the DNA.

The amplification mixtures are in “monotest” form in colour-identified PCR tubes of 0.2 – 0.5 ml.

All the PCR mixtures include primers marked at their 5’ end with 6-FAM fluorochrome, allowing automatic
analysis of fragments by capillary electrophoresis using GeneScan.

All amplifications can be carried out in the thermocycler using a single programme.

The enzyme Phire® Hot Start II DNA Polymerase is supplied in the kit.

Positive controls for clonal and polyclonal DNA are included.