IHC-Antibodies

Name: Antibody AFP(alpha-fetoprotein) clone EP209

Description and applications: Alpha-fetoprotein (AFP) is the most abundant plasma protein found in the human fetus. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. Alpha-Fetoprotein (AFP) is synthesized by the cells of the embryonic yolk sac, fetal liver and fetal intestinal tract. AFP levels decrease soon after birth. In abnormal tissues, expression of AFP has been demonstrated in hepatocellular carcinoma, hepatoid adenocarcinoma, germ cell tumors, particularly yolk sac tumor. The anti-AFP antibody may be useful for the identification of neoplastic liver diseases or yolk sac tumors.

Composition: anti-human AFP rabbit monoclonal antibody purified by protein affinity and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: A synthetic peptide corresponding to residues of human AFP protein

Name: Amyloid-A Monoclonal Antibody

Description and applications: Este anticuerpo reacciona con depósitos de amiloide de tipo AA, tanto la forma nativa como desnaturalizada de la proteína fibrilar. Este anticuerpo reacciona con depósitos de amiloide en todos los órganos y tejidos, así como con el precursor sérico de la proteína AA.

Composition: anti-Amyloid-A mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Name: CD23 Monoclonal Antibody clone SP23

Description and applications: The role of IgE immunoglobulins in the initiation and development of acute allergic processes is well known. These processes are generally initiated through its interaction with high affinity receptors for Fc portion (FceRI) located mainly on the surface of mast cells and basophil granulocytes where it regulates exocytosis phenomena and subsequent degranulation of mediators of hypersensitivity reactions type I. Furthermore, the immunoglobulin IgE also binds specifically to an integral membrane glycoprotein of 45- 55 kDa type 2 (321 aa) that does not belong to the immunoglobulin superfamily but to the selectins and are included under the name of low affinity receptor of IgE (FceRIIa+b / CD23). CD23 is expressed in a wide range of cell types, including B lymphocytes, eosinophils, granulocytes,  platelets, T and NK cells, dendritic reticulum cells and Langerhans cells. CD23 expression has been detected in neoplastic lymphoid cells of chronic lymphocytic leukemia, in a variable percentage of follicular lymphomas and in a small proportion of large B-cell lymphomas. Furthermore and although the CD23 molecule is produced by B cells, is adsorbed in a large amount on follicular dendritic reticulum cells thus is also used for the identification of its neoplasms (dendritic reticulum cell sarcoma).

Composition: anti-CD23 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Recombinant external domain of CD23 protein.

(Name: Mouse anti-CMV (Cytomegalovirus) cocktail of Monoclonal Antibodies (Clone DDG9/CCH2

Description and aplications: Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period.This antibody cocktail reacts with an immediate early antigen and an early CMV antigen. DDG9 is isotype IgG2a, kappa whilst CCH2 is isotype IgG1, kappa. The antibodies stain infected cells giving a nuclear staining pattern with an immediate early and an early antigen.

Composition: anti-CMV is a cocktail of two mouse monoclonal antibodies from tissue culture supernatant diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide.

.Immunogen: Cytomegalovirus-infected cell lysate

(Name: Mouse anti-human CA125 (Mucin-16) Monoclonal Antibody (Clone OC125

Description and applications: CA 125 (Cancer Antigen 125) is a mucin-like glycoprotein with a molecular weight greater than 200 kD. OC 125 recognizes an epitope that is considered to be a peptide in nature. Anti-CA-125 antibody reacts with epitheloid malignancies of the ovary, papillary serous carcinoma of the cervix, adenocarcinoma of the endometrium, clear cell adenocarcinoma of the bladder, and epitheloid mesothelioma. The antigen is formalin resistant, permitting the detection of ovarian cancer by immunohistochemistry, although serum assays for this protein are widely used to monitor ovarian cancer. CA 125 is located on the surface of ovarian tumor cells with essentially no expression in normal adult ovarian tissue. CA125 is also found in sera of patients with pancreatic, liver, colon, and other adenocarcinomas.1 These references are for presentation in vials of Low Density Polyethylene (LDPE) dropper. In case the products are used in automated stainers, a special reference is assigned as follows: – / L: Cylindrical screw-cap vials (QD-3 / L, QD-7 / L, QD-12 / L). – / N: Polygonal screw-cap vials (QD-3 / N, QD-7 / N, QD-12 / N). For different presentations (references / volumes) please contact the supplier. CA 125 is found in the epithelial cells of normal colon, gall bladder, mammary gland, and stomach. Normal breast, liver, skin, and spleen are usually negative. When used with other antibodies, CA 125 antibodies are useful for the separation of colonic carcinoma from ovarian endometroid carcinoma in the pelvis and distinguishing renal clear cell carcinoma from clear cell carcinoma of the ovary and other Mullerian tumors.

Composition: anti-human CA125 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Ovarian cancer cell line OVCA433 derived from patient with serous papillary cystadenocarcinoma.

.(Name:Rabbit anti-human Calretinin Monoclonal Antibody (Clone BSR235

Description and aplications: This antibody recognizes a protein of 31.5kDa, identified as Calretinin. Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain.  The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures.  In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas. However, occasional positivity for calretinin has been described in other tumors such as ameloblastomas, sex cord tumors of the ovary and testis.

Composition: anti-human Calretinin rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant full length mouse calretinin protein.

(Name: Rabbit anti-human Carbonic Anhydrase 9 Monoclonal Antibody (Clone EP161.

Description and applications: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and subcellular localization. Carbonic Anhydrase 9, a member of the carbonic anhydrase family, is thought to play a role in the regulation of cell proliferation in response to hypoxic conditions and may be involved in oncogenesis and tumor progression.Carbonic Anhydrase 9 (CA9) has a distinctive expression pattern in normal and cancer tissues. The most abundant expression of CA9 was found in normal mucosa of the stomach and gallbladder. Other normal tissues have lower or no expression. Relatively high levels of CA9 are expressed in carcinomas of the cervix, kidney, lung, breast and many other tumors. Most studies have shown that decreased CA9 levels are independently associated with poor survival. Low levels of CA9 maybe benefit more from adjuvant treatment than patients with high levels.

COMPOSITION: Anti-human Carbonic Anhydrase 9 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

IMMUNOGEN: A synthetic peptide corresponding to residues in the extracellular domain in human Carbonic Anhydrase 9 was used as an immunogen.

.(Name: Mouse anti-human Cytokeratin Monoclonal Antibody (Clone AE1

Description and applications: Due to the high homology among the different molecules, it is common for a monoclonal antibody to react with different types of cytokeratins; for example, anti-cytokeratin AE1 labels medium molecular weight acidic cytokeratins 10, 14, 15, 16, and 19, and low molecular weight acidic cytokeratin 19. Proteins generically called intermediate filaments, because they measure between 7 and 22 nm in diameter (i.e., with a size between actin, 5-7 nm, and tubulin, 22-25 nm), are part, along with actin and tubulin, of the vertebrate cytoskeleton. This superfamily is composed of six subfamilies of molecules with different tissue expression patterns. Cytokeratins constitute homology groups I and II, and in humans they are encoded by more than 49 different genes located in chromosomes 17 (I) and 12 (II). The nomenclature chosen in 1982 by Moll and Franke assigns numbers from 1 to 8 to type II cytokeratins (neutral or basic) and from 9 to 21 to type I cytokeratins (acidic). An analogous nomenclature for hair keratins has now been defined, with the addition of letters Ha and Hb to distinguish type I from type II. Structurally, cytokeratins share with the rest of intermediate filaments a central axisof 310 amino acid residues consisting of four highlypreserved α-helical domains (1A, 1B, 2A, and 2B) that define the type of intermediate filament that will be formed after assembly; these domains are separated by three non-helical linking regions (L1, L12, and L2) and two end domains which are highly different in, size and sequence (head, 1, and tail, 2), each with constant (E1/E2), variable (V1/V2), and homology (H1/H2) regions, the latter characteristic of type II keratins and absent in type I keratins. The major immunogenic properties and the main differences between each keratin class are found in the variable domains. Keratins are usually assembled into I/II heterodimers and are specifically co-expressed in pairs in each tissue. This antibody is widely reactive and labels almost all types of epithelia, thus being one of the best antibodies for the preliminary screening of neoplasms of an epithelial origin, and specially for the identification of malignant metastatic tumours of this origin. It does not react against all hepatocarcinomas, basal cell carcinomas, and adrenocortical carcinomas, and it partially reacts against renal cell carcinomas, small cell lung carcinomas, and large-cell carcinomasof the lung.

IMMUNOGEN: Epidermal cytokeratin.

.(Name: Mouse anti-human Cytokeratin Monoclonal Antibody (AE3

Description and applications: Due to the high homology among the different molecules, it is common for a monoclonal antibody to react with different types of cytokeratins; for example, anti-cytokeratin AE3 labels high molecular weight basic cytokeratins 1, 2, 3, 4, 5, and 6, as well as low molecular weight basic cytokeratins 7 and 8. Proteins generically called intermediate filaments, because they measure between 7 and 22 nm in diameter (i.e., with a size between actin, 5-7 nm, and tubulin, 22-25 nm), are part, along with actin and tubulin, of the vertebrate cytoskeleton. This superfamily is composed of six subfamilies of molecules with different tissue expression patterns. Cytokeratins constitute homology groups I and II, and in humans they are encoded by more than 49 different genes located in chromosomes 17 (I) and 12 (II). The nomenclature chosen in 1982 by Moll and Franke assigns numbers from 1 to 8 to type II cytokeratins (neutral or basic) and from 9 to 21 to type I cytokeratins (acidic). An analogous nomenclature for hair keratins has now been defined, with the addition of letters Ha and Hb to distinguish type I from type II. Structurally, cytokeratins share with the rest of intermediate filaments a central axis of 310 amino acid residues consisting of four highly preserved α- helical domains (1A, 1B, 2A, and 2B) that define the type of intermediate filament that will be formed after assembly; these domains are separated by three non-helical linking regions (L1, L12, and L2) and two end domains which are highly different in size and sequence (head, 1, and tail, 2), each with constant (E1/E2), variable (V1/V2), and homology (H1/H2) regions, the latter characteristic of type II keratins and absent in type I keratins. The major immunogenic properties and the main differences between each keratin class are found in the variable domains. Keratins are usually assembled into I/II heterodimers and are specifically co-expressed in pairs in each tissue. This antibody is highly reactive and positively stains almost all epithelial tissues and their neoplasms, including the skin, the esophagus, and the urinary bladder. Moreover, it labels pancreatic ducts and parenchymal cells and it does not stain mesenchymal cells. It does not react against all hepatocarcinomas. It does not cross-react with other types of filaments, including vimentin, desmin, and neurofilaments.

COMPOSITION: Anti-human Cytokeratin mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Mouse Anti-Human Keratin AE1-AE3 Cocktail Antibody clone AE1-AE3

Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins. This antibody, comprising a cocktail of AE1 and AE3 monoclonal antibodies, simultaneously recognizes keratins 10, 14, 15, 16 and 19 and 1, 2, 3, 4, 5, 6, 7 and 8, so that it is considered as one of the broadest spectrum reagents for the identification of epithelial origin in a given neoplasm. However, it should be borne in mind that besides hepatocarcinomas other tumors that often can be negative to the antibody AE1-AE3 are kidney, adrenal, prostate and neuroendocrine carcinomas. For these reasons the AE1-AE3 keratin antibody should be used in conjunction with other broad spectrum keratins.

Composition: anti-Keratin AE1-AE3 mouse antibody obtained obtained from mouse ascites by protein A chromatography in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The quantity of the active antibody in the sample is of approximately 4 μ/mL..

Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: Human epidermal cells