Name: CD3-PerCP- Cyanine 5.5, Clone UCHT-1

• Antibody CD3 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table above) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

The anti-CD recognizes the CD3 antigen present in T cells, and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of posttransplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. The T lymphocyte (CD3+) count is generally expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by flow cytometry based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure

REAGENT COMPOSITION

Purified monoclonal CD3 antibody conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: UCHT-1
• Isotypes: Mouse / IgG1
• Purification: Affinity chromatography
• Amount per vial: 50 or 200 tests (3 or 5 µl mAb to 106 cells).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD4-FITC, Clone RPA-T4

• Antibody CD4 is a monoclonal antibody (mAb) labelled with Fluorescein Isothicyanate, designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD4 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. CD4 mAb recognizes the CD4 antigen present in helper/inducer T cells (CD3+CD4+) and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. Individuals with HIV typically exhibit a steady decrease of helper/inducer T lymphocyte counts as the infection progresses. The CD4 antigen is also detected on some monocytes.

REAGENT COMPOSITION

Purified monoclonal CD4 antibody with Fluorescein Isothicyanate, is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Purification: Affinity chromatography
• Clone: RPA-T4
• Isotype: Mouse / IgG1
• Amount per vial: 200 tests (5 µl mAb to 106 cells).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD4-PE, Clone Edu-2

• Antibody CD4 is a monoclonal antibody (mAb) labelled with R-Phycoerythrin (PE), designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD4 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. CD4 mAb recognizes the CD4 antigen present in helper/inducer T cells (CD3+CD4+) and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. Individuals with HIV typically exhibit a steady decrease of helper/inducer T lymphocyte counts as the infection progresses. The CD4 antigen is also detected on some monocytes.

REAGENT COMPOSITION

Purified monoclonal CD4 antibody with R-Phycoerythrin, is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Purification: Affinity chromatography
• Clone: Edu-2
• Isotype: IgG2a
• Amount per vial: 200 tests (5 µl mAb to 106 cells).
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD4-PE-Cyanine5, Clone RPA-T4

• CD4 is a monoclonal antibody (mAb) labelled with different fluorochromes (see table), designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD4 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. CD4 mAb recognizes the CD4 antigen present in helper/inducer T cells (CD3+CD4+) and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. Individuals with HIV typically exhibit a steady decrease of helper/inducer T lymphocyte counts as the infection progresses. The CD4 antigen is also detected on some monocytes.

REAGENT COMPOSITION

Purified monoclonal CD4 antibody conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0,09% (m/v) sodium azide.

• Purification: Affinity chromatography
• Amount per vial: 50, 100 or 200 tests (3 or 5 µl mAb to 106 cells) (see table).
• Reagents are not considered sterile

Name: Flow Cytometry Antibody CD5-PerCP-Cyanine5.5, Clone L17F12

• Antibody CD5-PerCP-Cyanine5.5 is a monoclonal antibody (mAb) labelled with Peridin chlorophyll protein-Cyanine 5.5 (PerCPCyanine5.5), designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD5 antigen by Flow Cytometry (FC). CD5 is a useful marker for the identification of certain lymphoprolipherative syndromes when is used within an extended immunophenotyping panel. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

The CYT-5C2 mAb recognizes a 67 kDa antigen expressed by a majority of thymocytes and mature T cells and a subset of B cells. CD5 is expressed at low density on thymocytes and at high density on all mature T lymphocytes. The majority of T cell malignancies expresses CD5, and almost 85% of T cell acute lymphoblastic leukemias are CD5 positive. Further, CD5 is expressed at low density on a small subset of mature B lymphocytes, which is expanded in several autoimmune disorders as well as in some B-cell derived lymphoproliferative disorders such as chronic lymphocytic leukemia (CLL) and centrocytic leukemia.

REAGENT COMPOSITION

Purified monoclonal CD5 antibody conjugated with conjugated with PerCP-Cyanine5.5, supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: L17F12
• Isotype: Mouse / IgG2a
• Purification: Affinity chromatography
• Amount per vial: 50 tests (3 ml mAb to 106 cells) Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD7-APC (HULY-M2), Clone HULY-M2

• Antibody CD7-APC is a monoclonal antibody (mAb) labelled with the Allophycocyanine (APC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD7 antigen by flow cytometry (FC). The CD7 mAb is considered essential for the initial evaluation of T-cell acute lymphoblastic leukaemias (T-ALL) and T-cell chronic leukaemias together with a panel of other antibodies. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

CD7 is a 40KDa glycoprotein expressed on thymocytes, mature T cells, natural killer cells, pluripotent hematopoietic stem cells, and progenitors of lymphoid and myeloid cells. CD7 is one of the earliest T-cell-specific antigens to be expressed in lymphocytes, and the only early marker to persist throughout differentiation. Analysis of the expression of the CD7 antigen as a T cell line marker, in combination with those CD3 and CD5 antigens, is useful in the diagnosis of T cell proliferative syndromes such as T-ALL and T-prolymphocyte T cell leukaemias. The absence of expression of CD7, or its abnormal expression, can provide useful information in the case of Sezary’s syndrome or chronic lymphoid leukaemia. The CD7 antigen can be expressed by certain acute immature myeloid leukaemias.

REAGENT COMPOSITION

Purified monoclonal CD7 Antibody Allophycocyanine (APC) conjugated, is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: HULY-M2
• Isotype: Mouse / IgG2a
• Purification: Affinity chromatography
• Amount per 1 ml vial: 200 tests (5 ml mAb to 106 cells)
• Reagents are not considered sterile.