Flocytometry Antibodies

Name: Flow Cytometry Antibody CD20-FITC, Clone 2H7

• Antibody Anti-human CD20 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD20 antigen by Flow Cytometry (FC). This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). B lymphocytes are the producers of antibodies, they mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. CD20 can be considered a pan-B cell antigen, as it is expressed on the surface of all mature B-lymphocytes but not in secreting plasma cells. Anti-human CD20 reagent recognizes CD20 antigen present on B cells and can therefore be used in lymphocytes immunophenotyping. Antibodies to CD20 are widely applied in the characterization and follow-up of acute and chronic lymphoproliferative disorders. Pacific BlueTM is excited with the violet laser (405nm) and emits at 550nm. This fluorochrome provides maximum resolution and narrow emission peaks, which results in little spectral overlap and minimal compensation requirements. In multicolor panel it is recommended the use of this fluorochrome combined with Orange Cytognos 515, which is also excited with the violet laser and emits at 515nm.

REAGENT COMPOSITION

Purified anti-human CD20 mAb conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 0,09% (m/v) sodium azide

• Amount per vial: 100 or 200 tests (5 µl mAb per determination) (see table above).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD20-Pacific Blue™, Clone 2H7

• Antibody Anti-human CD20 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD20 antigen by Flow Cytometry (FC). This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). B lymphocytes are the producers of antibodies, they mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. CD20 can be considered a pan-B cell antigen, as it is expressed on the surface of all mature B-lymphocytes but not in secreting plasma cells. Anti-human CD20 reagent recognizes CD20 antigen present on B cells and can therefore be used in lymphocytes immunophenotyping. Antibodies to CD20 are widely applied in the characterization and follow-up of acute and chronic lymphoproliferative disorders. Pacific BlueTM is excited with the violet laser (405nm) and emits at 550nm. This fluorochrome provides maximum resolution and narrow emission peaks, which results in little spectral overlap and minimal compensation requirements. In multicolor panel it is recommended the use of this fluorochrome combined with Orange Cytognos 515, which is also excited with the violet laser and emits at 515nm.

REAGENT COMPOSITION

Purified anti-human CD20 mAb conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 0,09% (m/v) sodium azide

• Amount per vial: 100 or 200 tests (5 µl mAb per determination) (see table above).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD20-PE, Clone Bly1

• Antibody Anti-human CD20 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD20 antigen by Flow Cytometry (FC). This reagent must be used by FC qualified personal. CD20 can be considered a pan-B cell antigen, as it is expressed on the surface of all mature B-lymphocytes but not in secreting plasma cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). B lymphocytes are the producers of antibodies, they mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. Anti-human CD20 reagent recognizes CD20 antigen present on B-cells,and can therefore be used in lymphocytes immunophenotyping. Antibodies to CD20 are widely applied in the characterization and follow-up of acute and chronic lymphoproliferative disorders.

REAGENT COMPOSITION

Purified anti-human CD20 mAb conjugated with different fluorochromes (see table above) is supplied in phosphate-buffered saline (PBS) containing ≤0,09% (m/v) sodium azide

• Amount per vial: 200 tests (5 µl mAb per determination) (see table above).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD22-FITC, Clone B-ly8

• Antibody CD22-FITC is a monoclonal antibody (mAb) labelled with Fluorescein Isothiocyanate (FITC) designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of CD22 antigen-expressing cells.

SUMMARY AND EXPLANATION

Flow Cytometry (FC) is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells. CD22 antigen (also named SIGLEC-2) is a type I transmembrane glycoprotein present on the surface of mature B and activated B cells (but not on plasma cells), and in the cytoplasm of pro-B and pre-B cells. CD22 has been reported to participate in B-cell activation via surface Ig and also as an adhesion molecule. CD22 is used in flow cytometry for the immunophenotyping of B cell lineage leukemias and lymphomas. Aberrant CD22 expression is useful for differentiating neoplastic from benign B- cells. CD22 is overexpressed in hairy cell leukemia and splenic marginal zone lymphoma, whereas is under expressed or shows an asynchronous expression in other B-chronic lymphoproliferative disease groups, including chronic lymphocytic leukemia, prolymphocytic leukemia, mantle-cell lymphoma, follicular lymphoma and lymphoplasmacytic lymphoma.

REAGENT COMPOSITION

The purified monoclonal CD22 Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: B-ly8.
• Isotype: IgG1.
• Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD22-PE, Clone MEM-01

• Antibody CD22-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD22 antigen-expressing cells. This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). CD22 antigen (also named SIGLEC-2) is a type I transmembrane glycoprotein present on the surface of mature B and activated B cells (but not on plasma cells), and in the cytoplasm of pro-B and pre-B cells. CD22 has been reported to participate in B-cell activation via surface Ig and also as an adhesion molecule. CD22 is used in flow cytometry for the immunophenotyping.

REAGENT COMPOSITION

The purified monoclonal CD22 Antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.09% sodium azide.

• Clone: MEM-01.
• Isotype: IgG1.
• Amount per 1 mL vial: 200 tests (5 mL mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD23-FITC, Clone EBVCS-5

• Antibody CD23-FITC is a monoclonal antibody (mAb) labelled with Fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD23 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression.

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). CD23 antigen is a type II integral membrane glycoprotein. It is a member of the Ig family, expressed on most mature B cells, mantle zone B cells (but not in proliferating germinal center cells), follicular dendritic cells, monocytes, eosinophils, Langerhans cells, and a subset of T cells (10-15% of tonsillar T cells). CD23 is the low affinity receptor for IgE and plays important roles in the regulation of IgE responses; it is responsible for interaction with many of its known ligands, including IgE, CD21, major histocompatibility complex (MHC) class II and integrins. Anti CD23 antibody is used in flow cytometry for the immunophenotyping of B cell lineage leukemias and lymphomas. The diagnostic and prognostic utility of CD23 expression has been widely studied in B cell chronic lymphocytic leukemia. CD23 is overexpressed in some B-chronic lymphoproliferative diseases, mantle cell lymphoma, small cell lymphocytic lymphoma, follicular dendritic cell sarcoma and plasmacytomas with abnormalities on chromosome 11, whereas is generally absent from follicular lymphoma cells and acute lymphoblastic leukaemia cells.

REAGENT COMPOSITION

The purified monoclonal CD23 Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: EBVCS-5
• Isotype: IgG1.
• Amount per vial: 100 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD25-APC, Clone M-A251

• Antibody CD25-APC is a monoclonal antibody (mAb) labelled with allophycocyanine (APC) designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of CD25 antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

CD25 antigen is part of interleukin-2 receptors. These receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2. The 55 kDa α chain is called CD25. The α chain alone, the βγ heterodimer, and the αβγ heterotrimer form the low-, intermediate- and high affinity IL-2R, respectively. The intermediate- and high affinity IL-2R play a role in intracellular signal transduction. CD25 is strongly expressed on the surface of T cells activated by antigens, mitogens (phytohemagglutinin A (PHA), concanavalin A (Con-A), pokeweed mitogen (PWM)), Staphylococcus aureus, viruses (HTLV-I, HTLV-II, Epstein-Barr virus), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide. In normal peripheral blood a variable staining of lymphoid cells (about 3-25%) is observed.

REAGENT COMPOSITION

The purified monoclonal CD25 antibody conjugated with allophycocyanine (APC) supplied in phosphate buffered saline with 0,09% sodium azide and 0.2% (w/v) bovine serum albumin (BSA).

• Clone: M-A251.
• Isotype: IgG1.
• Amount per vial: 100 tests 5 µl mAb per determination.
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD25-FITC, Clone MEM-181

• Antibody Anti-human CD25 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD25 antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

CD25 antigen is part of interleukin-2 receptors. These receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2. The 55 kDa α chain is called CD25. The α chain alone, the βγ heterodimer, and the αβγ heterotrimer form the low-, intermediate- and high affinity IL-2R, respectively. The intermediate- and high affinity IL-2R play a role in intracellular signal transduction. CD25 is strongly expressed on the surface of T cells activated by antigens, mitogens (phytohemagglutinin A (PHA), concanavalin A (Con-A), pokeweed mitogen (PWM)), Staphylococcus aureus, viruses (HTLV-I, HTLV-II, Epstein-Barr virus), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide. In normal peripheral blood a variable staining of lymphoid cells (about 3-25%) is observed.

REAGENT COMPOSITION

Purified anti-human CD25 mAb conjugated with different fluorochromes (see table above) is supplied in phosphate-buffered saline (PBS) containing 0,09% (m/v) sodium azide and 0.2% (w/v) bovine serum albumin (BSA).

• Clone: MEM-181.
• Isotype: Mouse/IgG1.
• Amount per vial: 1ml/ 100 tests (10 µl mAb per determination) (see table above).
• Purification: Affinity chromatography.
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD25-PE, Clone MEM-181

• Anti-human CD25 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD25 antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

CD25 antigen is part of interleukin-2 receptors. These receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2. The 55 kDa α chain is called CD25. The α chain alone, the βγ heterodimer, and the αβγ heterotrimer form the low-, intermediate- and high affinity IL-2R, respectively. The intermediate- and high affinity IL-2R play a role in intracellular signal transduction. CD25 is strongly expressed on the surface of T cells activated by antigens, mitogens (phytohemagglutinin A (PHA), concanavalin A (Con-A), pokeweed mitogen (PWM)), Staphylococcus aureus, viruses (HTLV-I, HTLV-II, Epstein-Barr virus), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide. In normal peripheral blood a variable staining of lymphoid cells (about 3-25%) is observed.

REAGENT COMPOSITION

Purified anti-human CD25 mAb conjugated with different fluorochromes (see table above) is supplied in phosphate-buffered saline (PBS) containing 0,09% (m/v) sodium azide and 0.2% (w/v) bovine serum albumin (BSA).

• Clone: MEM-181.
• Isotype: Mouse/IgG1.
• Amount per vial: 1ml/ 100 tests (10 µl mAb per determination) (see table above).
• Purification: Affinity chromatography.
• Reagent is considered non-sterile.