Flocytometry Antibodies

Name: Flow Cytometry Antibody CD35-FITC, Clone E11

• Antibody CD35-FITC is a monoclonal antibody (mAb) labelled with Fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD35 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. CD35, also known as complement receptor 1 (CR1), is a N-glycosylated member of the RCA (regulators of complement activation) family of proteins. It is expressed by erythrocytes, granulocytes, monocytes, dendritic cells, B cells and some NK cells. CD35 is also expressed leukemic cells in a subset of AML (acute myeloid leukemia) patients. CD35 has been selected by EuroFlow consortium in its AML panel .

REAGENT COMPOSITION

The purified monoclonal CD35 Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: E11
• Isotype: IgG1.
• Amount per vial: 100 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD38-PE, Clone T16

• Antibody CD38-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for Flow Cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of human CD38 antigen-expressing cells. This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

CD38 antigen is a type II transmembrane glycoprotein expressed at variable levels on the majority of hematopoietic cells (during early differentiation and activation) and in some non-hematopoietic tissues. It is expressed at high levels on plasma cells (CD38+++) and, due to its strong reactivity, CD38 is considered a “specific plasma cell marker”; but it is also present on thymocytes, activated T cells, NKs monocytes, and basophils.. CD38 has been reported to play a role as regulator (positive and negative) of cell activation and proliferation, and to be involved in adhesion between lymphocytes and endothelial cells. CD38 antibody is used in FC for diagnosis and monitoring of plasma cell disorders (e.g. multiple myeloma, MGUS, plasma cell leukemia, etc.). The simultaneous assessment of the expression of CD38 and CD138 represents the best combination of markers for the identification of plasma cells. Plasma cells (CD38+++) are expressed as a percentage of leucocytes present in a sample. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure and the combination of markers used to identify the target cell population.

REAGENT COMPOSITION

The purified monoclonal CD38 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.09% (m/v) sodium azide.

• Clone: T16.
• Isotype: IgG1.
• Size: 200 tests (1 ml per vial).
• Usage: 5 µl mAb per determination.
• Reagent is considered non-sterile

Name: Flow Cytometry Antibody CD38-Pure (RUO), Clone LD38

• Antibody CD38 is a monoclonal antibody (mAb) designed for flow cytometry (FC) use as direct (or indirect for the CD38-PURE) immunofluorescence reagent for the identification and enumeration of CD38 antigen-expressing cells. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

CD38 antigen is a type II transmembrane glycoprotein expressed at variable levels on the majority of hematopoietic cells (during early differentiation and activation) and in some non-hematopoietic tissues. It is expressed at high levels on plasma cells (CD38+++) and, due to its strong reactivity, CD38 is considered a “specific plasma cell marker”; but it is also present on thymocytes and activated T cells. CD38 has been reported to play a role as regulator (positive and negative) of cell activation and proliferation, and to be involved in adhesion between lymphocytes and endothelial cells. The simultaneous assessment of the expression of CD38 and CD138 represents the best combination of markers for the identification of plasma cells. Plasma cells (CD38+++) are expressed as a percentage of leucocytes present in a sample. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

CD38 antibody is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0,09% (m/v) sodium azide.

• Clone: LD38.
• Isotype: Mouse / IgG1.
• Amount per vial: 50, 100 or 200 tests (3, 5 or 10 µL mAb per determination (see table above)).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD38-Pure, Clone EBVCS-5

• Antibody CD38 is a monoclonal antibody (mAb) designed for flow cytometry (FC) use as direct (or indirect for the CD38-PURE) immunofluorescence reagent for the identification and enumeration of CD38 antigen-expressing cells. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

CD38 antigen is a type II transmembrane glycoprotein expressed at variable levels on the majority of hematopoietic cells (during early differentiation and activation) and in some non-hematopoietic tissues. It is expressed at high levels on plasma cells (CD38+++) and, due to its strong reactivity, CD38 is considered a “specific plasma cell marker”; but it is also present on thymocytes and activated T cells. CD38 has been reported to play a role as regulator (positive and negative) of cell activation and proliferation, and to be involved in adhesion between lymphocytes and endothelial cells. CD38 antibody is used in FC for diagnosis and monitoring of plasma cell disorders (e.g. multiple myeloma, MGUS, plasma cell leukemia, etc.). The simultaneous assessment of the expression of CD38 and CD138 represents the best combination of markers for the identification of plasma cells. Plasma cells (CD38+++) are expressed as a percentage of leucocytes present in a sample. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

CD38 antibody is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0,09% (m/v) sodium azide.

• Clone: LD38.
• Isotype: Mouse / IgG1.
• Amount per vial: 50, 100 or 200 tests (3, 5 or 10 µl mAb per determination (see table above)).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD43-APC-C750™, Clone TP1/36

• Antibody CD43-APC-C750^TM is a monoclonal antibody (mAb) labelled with the tandem allophycocyanine-C750 (APC-C750^TM) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD43 antigen by Flow Cytometry (FC).

SUMMARY AND EXPLANATION

FC is a powerful tool for the analytical and quantitative characterization of cells which provides rapid, quantitative and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cells in liquid suspension that have been incubated with fluorescently labeled antibodies directed against specific cellular proteins. The relative fluorescence intensity of the positive cells indicates the amount of antibody bound to specific binding sites on the cells, and therefore provides a relative measure of antigen expression. CYT-43AC750 mAb recognizes the CD43 antigen, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialo glycoprotein and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. CD43 exerts dual function in cell adhesion and anti-adhesion, lectin binding, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors .During T-cell activation is actively removed from the T-Cell APC (antigen presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. The expression of CD43 is found on most leukocytes, except resting B lymphocytes. APC-C750^TM is a tandem dye with a maximum emission peak at 779nm, which grants bright signal, low unspecific noise and high photostability. When excited by light from a red laser, the APC fluorochrome can transfer energy to C750 molecule, which then emits at a longer wavelength. It is recommended to use a 780/60 nm bandpass filter along with a red sensitive detector to use in conjunction antibodies conjugated with APC and APC-C750^TM .

REAGENT COMPOSITION

Purified monoclonal CD43 antibody conjugated with the tandem allophycocyanine-C750 (APC-C750TM), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.

• Clone: TP1/36
• Isotype: IgG1
• Amount per vial: 50 tests (3µl mAb per determination)
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD43-APC-C750™, Clone TP1/36

• Antibody CD43-APC-C750^TM is a monoclonal antibody (mAb) labelled with the tandem allophycocyanine-C750 (APC-C750^TM) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD43 antigen by Flow Cytometry (FC).

SUMMARY AND EXPLANATION

FC is a powerful tool for the analytical and quantitative characterization of cells which provides rapid, quantitative and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cells in liquid suspension that have been incubated with fluorescently-labeled antibodies directed against specific cellular proteins. The relative fluorescence intensity of the positive cells indicates the amount of antibody bound to specific binding sites on the cells, and therefore provides a relative measure of antigen expression. CYT-43AC750 mAb recognizes the CD43 antigen, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. CD43 exerts dual function in cell adhesion and anti-adhesion, lectin binding, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors .During T-cell activation is actively removed from the T-Cell APC (antigen presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. The expression of CD43 is found on most leukocytes, except resting B lymphocytes. APC-C750TM is a tandem dye with a maximum emission peak at 779nm, which grants bright signal, low unspecific noise and high photostability. When excited by light from a red laser, the APC fluorochrome can transfer energy to C750 molecule, which then emits at a longer wavelength. It is recommended to use a 780/60 nm bandpass filter along with a red sensitive detector to use in conjunction antibodies conjugated with APC and APC-C750^TM .

REAGENT COMPOSITION

Purified monoclonal CD43 antibody conjugated with the tandem allophycocyanine-C750 (APC-C750TM), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.

• Clone: TP1/36
• Isotype: IgG1
• Amount per vial: 50 tests (3µl mAb per determination)
• Reagents are not considered sterile.

Name: Flow Cytometry Antibody CD43-FITC, Clone DFT1

• Antibody CD43-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothicyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD43 antigen by Flow Cytometry (FC).

SUMMARY AND EXPLANATION

FC is a powerful tool for the analytical and quantitative characterization of cells which provides rapid, quantitative and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cells in liquid suspension that have been incubated with fluorescently-labeled antibodies directed against specific cellular proteins. The relative fluorescence intensity of the positive cells indicates the amount of antibody bound to specific binding sites on the cells, and therefore provides a relative measure of antigen expression. CYT-43F2 mAb recognizes the CD43 antigen, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. CD43 exerts dual function in cell adhesion and anti-adhesion, lectin binding, as well as co-stimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors .During T-cell activation is actively removed from the T-Cell APC (antigen presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. The expression of CD43 is found on most leukocytes, except resting B lymphocytes.

REAGENT COMPOSITION

Purified monoclonal CD43 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with ≤0.09% (m/v) sodium azide.

• Clone: DFT1
• Isotype: IgG1
• Amount per vial: 200 tests (5µl mAb per determination)
• Reagents are not considered sterile.