آنتی بادی مونوکلونال فلوسایتومتری CD43-APC ، کلون TP1/36

Name: Flow Cytometry Antibody CD43-APC-C750™, Clone TP1/36

• Antibody CD43-APC-C750^TM is a monoclonal antibody (mAb) labelled with the tandem allophycocyanine-C750 (APC-C750^TM) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD43 antigen by Flow Cytometry (FC).

SUMMARY AND EXPLANATION

FC is a powerful tool for the analytical and quantitative characterization of cells which provides rapid, quantitative and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cells in liquid suspension that have been incubated with fluorescently labeled antibodies directed against specific cellular proteins. The relative fluorescence intensity of the positive cells indicates the amount of antibody bound to specific binding sites on the cells, and therefore provides a relative measure of antigen expression. CYT-43AC750 mAb recognizes the CD43 antigen, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialo glycoprotein and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. CD43 exerts dual function in cell adhesion and anti-adhesion, lectin binding, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors .During T-cell activation is actively removed from the T-Cell APC (antigen presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. The expression of CD43 is found on most leukocytes, except resting B lymphocytes. APC-C750^TM is a tandem dye with a maximum emission peak at 779nm, which grants bright signal, low unspecific noise and high photostability. When excited by light from a red laser, the APC fluorochrome can transfer energy to C750 molecule, which then emits at a longer wavelength. It is recommended to use a 780/60 nm bandpass filter along with a red sensitive detector to use in conjunction antibodies conjugated with APC and APC-C750^TM .

REAGENT COMPOSITION

Purified monoclonal CD43 antibody conjugated with the tandem allophycocyanine-C750 (APC-C750TM), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.

• Clone: TP1/36
• Isotype: IgG1
• Amount per vial: 50 tests (3µl mAb per determination)
• Reagents are not considered sterile.