FITC Tech Kits

Anti-Human C1q

DESCRIPTION AND APPLICATIONS :C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE).

This antibody should be used in conjunction with a panel of antibodies to aid in the identification of C1q fragment in target tissue (e.g., in the diagnosis of renal or dermal pathologies).

The interpretation of the results should be always corroborated with those of external controls and integrated with the morphological and clinical aspects for the final diagnosis

COMPOSITION: Anti-human Sheep Antibody to C1q. Fluorescein Conjugated Polyclonal antibody purified from serum and prepared in buffer with carrier protein and preservative for stabilization.

IMMUNOGEN: Human C1q, purified from plasma

Anti-Human C3c

DESCRIPTION AND APPLICATIONS: The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes. This antibody should be used in conjunction with a panel of antibodies to aid in the identification of C3c in target tissue (e.g., in the diagnosis of renal or dermal pathologies). The interpretation of the results should be always corroborated with those of external controls and integrated with the morphological and clinical aspects for the final diagnosis

COMPOSITION Anti-human Sheep Antibody to C3c. Fluorescein Conjugated Polyclonal antibody purified from serum and prepared in buffer with carrier protein and preservative for stabilization.

IMMUNOGEN: Human C3c purified from plasma

 Anti-Human C4

DESCRIPTION AND APPLICATIONS: This antibody reacts with human C4, C4b, and C4c but does not react with the C4d epitope. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.

This antibody should be used in conjunction with a panel of antibodies to aid in the identification of C4c fragment in target tissue (e.g., in the diagnosis of renal or dermal pathologies).

The interpretation of the results should be always corroborated with those of external controls and integrated with the morphological and clinical aspects for the final diagnosis.

 COMPOSITION

Anti-human Rabbit Antibody to C4c. Fluorescein Conjugated Polyclonal antibody purified from serum and prepared in buffer with carrier protein and preservative for stabilization

Immunogen

 C4c Complement isolated from complement-activated human serum.

 Anti-Human Gamma Heavy Chain (IgG)

DESCRIPTION AND APPLICATIONS: Goat anti Human IgG antibody reacts with the heavy chain of human IgG and has been cross-absorbed with human IgM, IgD and IgA, but may recognize IgG from other species.

This antibody should be used in conjunction with a panel of antibodies to aid in the identification of immunoglobulin G in target tissue (e.g., in the diagnosis of renal or dermal pathologies).

The interpretation of the results should be always corroborated with those of external controls and integrated with the morphological and clinical aspects for the final diagnosis.

COMPOSITION

Anti-human Goat Antibody to Gamma Heavy Chain (IgG). Fluorescein Conjugated Polyclonal antibody purified from serum and prepared in buffer with carrier protein and preservative for stabilization

IMMUNOGEN: Normal human IgG and human IgG paraproteins.

 Anti-Human Mu Heavy Chain (IgM)

 DESCRIPTION AND APPLICATIONS: Mouse anti Human IgM antibody, clone 47.2.E9 reacts with human IgM and recognizes the Mu chain.

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

This antibody should be used in conjunction with a panel of antibodies to aid in the identification of immunoglobulin M in target tissue (e.g., in the diagnosis of renal or dermal pathologies).

The interpretation of the results should be always corroborated with those of external controls and integrated with the morphological and clinical aspects for the final diagnosis.

 COMPOSITION

Anti-human Mouse Antibody to Mu Heavy Chain (IgM). Fluorescein Conjugated Monoclonal antibody purified from serum and prepared in buffer with carrier protein and preservative for stabilization.

 IMMUNOGEN: Native IgM.

 Anti-Human Kappa Light Chain

DESCRIPTION AND APPLICATIONS: Anti-Human Kappa Light Chain (Bound and Free)−FITC antibody is specific for human kappa light chain when tested against purified bound kappa light chain (human IgA, IgG, and IgM) and free kappa light chain (Bence Jones Kappa myeloma protein). The conjugate shows no reaction with bound or free lambda light chains. Mammalian antibodies contain one of two types of light chain, called kappa or lamba. Each chain contains a constant and a variable domain. Mammalian antibodies contain either two kappa or two lambda light chains.

This antibody should be used in conjunction with a panel of antibodies to aid in the identification of Kappa light chain of immunoglobulin in target tissue (e.g., in the diagnosis of renal or dermal pathologies). The interpretation of the results should be always corroborated with those of external controls and

COMPOSITION

Anti-human Goat Antibody to Kappa Light Chain. Fluorescein Conjugated Polyclonal antibody purified from serum and prepared in buffer with carrier protein and preservative for stabilization

 IMMUNOGEN: Human kappa chains.